CDK5 suppresses the metastasis of gastric cancer cells by interacting with and regulating PP2A.
Cell Line, Tumor
Cell Movement
/ drug effects
Cell Proliferation
/ drug effects
Cyclin-Dependent Kinase 5
/ metabolism
Down-Regulation
Female
Follow-Up Studies
Humans
Male
Middle Aged
Neoplasm Invasiveness
/ pathology
Okadaic Acid
/ pharmacology
Prognosis
Protein Binding
Protein Phosphatase 2
/ antagonists & inhibitors
Stomach
/ pathology
Stomach Neoplasms
/ mortality
Journal
Oncology reports
ISSN: 1791-2431
Titre abrégé: Oncol Rep
Pays: Greece
ID NLM: 9422756
Informations de publication
Date de publication:
Feb 2019
Feb 2019
Historique:
received:
16
03
2018
accepted:
16
10
2018
pubmed:
16
11
2018
medline:
1
2
2019
entrez:
16
11
2018
Statut:
ppublish
Résumé
Several previous studies have demonstrated that cyclin‑dependent kinase (CDK)‑5 expression serves an important role in promoting the development of malignant tumours. We have previously reported that CDK5 suppresses gastric tumourigenesis. The aim of the present study was to investigate the mechanistic basis of CDK5. The results of immunoprecipitation and western blot analysis demonstrated that CDK5 could interact with serine/threonine‑protein phosphatase 2A (PP2A). The use of an inhibitor of PP2A in CDK5‑overexpressing gastric cancer (GC) cell lines antagonized CDK5‑mediated suppression in GC cells. Further analysis revealed that PP2A expression was downregulated in GC and patients with low levels of PP2A had worse survival outcomes than those with high levels of PP2A (P=0.035). Therefore, the present study provided a novel mechanism for CDK5‑mediated tumour suppression, suggesting that CDK5 may be an attractive target for future therapeutic strategies for treating GC. In addition, low levels of PP2A may indicate a tendency for poor prognosis in patients with GC.
Identifiants
pubmed: 30431123
doi: 10.3892/or.2018.6860
pmc: PMC6312987
doi:
Substances chimiques
Okadaic Acid
1W21G5Q4N2
Cyclin-Dependent Kinase 5
EC 2.7.11.1
CDK5 protein, human
EC 2.7.11.22
Protein Phosphatase 2
EC 3.1.3.16
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
779-788Références
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