Antimicrobial activity of bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus: from shake flasks to bioreactor.


Journal

Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234

Informations de publication

Date de publication:
Feb 2019
Historique:
received: 16 10 2018
accepted: 10 11 2018
pubmed: 19 11 2018
medline: 3 7 2019
entrez: 19 11 2018
Statut: ppublish

Résumé

Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria (LAB), which have a large spectrum of action against spoilage bacteria and foodborne pathogens. However, when not entirely characterized, they are alternatively called bacteriocin-like inhibitory substances (BLIS). Pediococcus pentosaceus ATCC 43200 grew and produced BLIS optimally when cultivated anaerobically in bioreactor for 24 h at 30 °C and 200 rpm in De Man, Rogosa and Sharp (MRS) medium supplemented with 1.5% peptone. Under such optimal conditions, the cell mass concentration (3.41 g/L) was 66% higher, the generation time (1.28 h) 38% shorter and the BLIS activity against different indicator strains significantly higher than in MRS medium without any supplement taken as a control, and the exponential phase started 4 h before. The agar diffusion method showed BLIS inhibition halos against LAB strains with diameter in the range 11.0-19.5 mm and specific areas between 377.1 and 2654.6 mm

Identifiants

pubmed: 30448894
doi: 10.1007/s11033-018-4495-y
pii: 10.1007/s11033-018-4495-y
doi:

Substances chimiques

Anti-Bacterial Agents 0
Anti-Infective Agents 0
Bacteriocins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

461-469

Subventions

Organisme : FAPESP
ID : 2016/06284-9
Organisme : CAPES
ID : Financial code 001

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Auteurs

Pamela Oliveira de Souza de Azevedo (POS)

Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, Brazil.

Attilio Converti (A)

Department of Civil, Chemical and Environmental Engineering, Pole of Chemical Engineering, Via Opera Pia 15, 16145, Genoa, Italy.

Martin Gierus (M)

Department of Agrobiotechnology, Institute of Animal Nutrition, Livestock Products, and Nutrition Physiology (TTE), IFA-Tulln, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.

Ricardo Pinheiro de Souza Oliveira (RPS)

Department of Biochemical and Pharmaceutical Technology, University of São Paulo, São Paulo, SP, Brazil. rpsolive@usp.br.

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Classifications MeSH