Lysophospholipid G protein-coupled receptor binding parameters as determined by backscattering interferometry.
binding assay
endocannabinoid
lipid
molecular interaction
optical measurement
phospholipids
Journal
Journal of lipid research
ISSN: 1539-7262
Titre abrégé: J Lipid Res
Pays: United States
ID NLM: 0376606
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
17
09
2018
revised:
13
11
2018
pubmed:
23
11
2018
medline:
11
2
2020
entrez:
23
11
2018
Statut:
ppublish
Résumé
Lysophosphatidic acid (LPA) activates cognate G protein-coupled receptors (GPCRs) to initiate biological signaling cascades. Lysophospholipid (LP) receptor binding properties remain incompletely assessed because of difficulties with ligand lipophilicity and lipid "stickiness." These inherent attributes produce high levels of nonspecific binding within cell-membrane preparations used to assess GPCRs, as has been shown in classical binding assays using radiolabeled ligands, making accurate measurements of lipid binding kinetics difficult to achieve. Backscattering interferometry (BSI) is an optical technology that measures molecular binding interactions by reporting changes in the refractive index of a solution after binding events. Here, we report the use of BSI to assess LPA
Identifiants
pubmed: 30463988
pii: S0022-2275(20)32678-X
doi: 10.1194/jlr.D089938
pmc: PMC6314248
doi:
Substances chimiques
Ligands
0
Lysophospholipids
0
Receptors, G-Protein-Coupled
0
lysophosphatidic acid
PG6M3969SG
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
212-217Subventions
Organisme : NINDS NIH HHS
ID : R01 NS084398
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS103940
Pays : United States
Informations de copyright
Copyright © 2019 Mizuno et al.
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