Cryobiopsy with endobronchial ultrasonography using a guide sheath for peripheral pulmonary lesions and DNA analysis by next generation sequencing and rapid on-site evaluation.
Aged
Aged, 80 and over
Bronchoscopy
/ methods
Endosonography
/ methods
Female
High-Throughput Nucleotide Sequencing
/ methods
Humans
Image-Guided Biopsy
/ methods
Lung
/ pathology
Lung Neoplasms
/ diagnosis
Male
Middle Aged
Predictive Value of Tests
Prospective Studies
Sensitivity and Specificity
Sequence Analysis, DNA
/ methods
Cryobiopsy
DNA-sequencing
EBUS-GS
PPLs
ROSE
Journal
Respiratory investigation
ISSN: 2212-5353
Titre abrégé: Respir Investig
Pays: Netherlands
ID NLM: 101581124
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
12
06
2018
revised:
10
10
2018
accepted:
23
10
2018
pubmed:
25
11
2018
medline:
8
6
2019
entrez:
25
11
2018
Statut:
ppublish
Résumé
The purpose of this study was to evaluate the diagnostic accuracy of Cryo with endobronchial ultrasonography using a guide sheath (EBUS-GS) for peripheral pulmonary lesions (PPLs) to assess the volume of specimen, determine DNA sequencing analysis, and evaluate the utility of rapid on-site evaluation (ROSE). Out of 30 patients assessed for eligibility, 23 were enrolled in this prospective study. The histological diagnostic yield of Cryo was evaluated and the volume was compared to that of trans-bronchial biopsy (TBB). DNA analysis of Cryo was performed using next generation sequencing (NGS). ROSE was compared with the final diagnosis. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy rate was 85%, 100%, 100%, 50%, 87% for Cryo and 80%, 100%, 100%, 42.9%, 82.6% for TBB, respectively. The mean volume was 0.078 cm Cryo with EBUS-GS for PPLs is a safe and potentially useful diagnostic strategy. It has a high diagnostic yield, and provides significantly larger specimens than TBB. It also provides high quantity and quality of DNA for NGS and high concordance rate between ROSE and the final diagnosis.
Sections du résumé
BACKGROUND
BACKGROUND
The purpose of this study was to evaluate the diagnostic accuracy of Cryo with endobronchial ultrasonography using a guide sheath (EBUS-GS) for peripheral pulmonary lesions (PPLs) to assess the volume of specimen, determine DNA sequencing analysis, and evaluate the utility of rapid on-site evaluation (ROSE).
METHODS
METHODS
Out of 30 patients assessed for eligibility, 23 were enrolled in this prospective study. The histological diagnostic yield of Cryo was evaluated and the volume was compared to that of trans-bronchial biopsy (TBB). DNA analysis of Cryo was performed using next generation sequencing (NGS). ROSE was compared with the final diagnosis.
RESULTS
RESULTS
The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy rate was 85%, 100%, 100%, 50%, 87% for Cryo and 80%, 100%, 100%, 42.9%, 82.6% for TBB, respectively. The mean volume was 0.078 cm
CONCLUSIONS
CONCLUSIONS
Cryo with EBUS-GS for PPLs is a safe and potentially useful diagnostic strategy. It has a high diagnostic yield, and provides significantly larger specimens than TBB. It also provides high quantity and quality of DNA for NGS and high concordance rate between ROSE and the final diagnosis.
Identifiants
pubmed: 30470645
pii: S2212-5345(18)30111-4
doi: 10.1016/j.resinv.2018.10.006
pii:
doi:
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
150-156Informations de copyright
Copyright © 2018 The Japanese Respiratory Society. Published by Elsevier B.V. All rights reserved.