BICELLULAR POLLEN 1 is a modulator of DNA replication and pollen development in Arabidopsis.


Journal

The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884

Informations de publication

Date de publication:
04 2019
Historique:
received: 18 07 2018
accepted: 19 11 2018
pubmed: 30 11 2018
medline: 28 2 2020
entrez: 29 11 2018
Statut: ppublish

Résumé

During male gametogenesis in Arabidopsis, the haploid microspore undergoes an asymmetric division to produce a vegetative and a generative cell, the latter of which continues to divide symmetrically to form two sperms. This simple system couples cell cycle with cell fate specification. Here we addressed the role of DNA replication in male gametogenesis using a mutant bicellular pollen 1 (bice1), which produces bicellular, rather than tricellular, pollen grains as in the wild-type plant at anthesis. The mutation prolonged DNA synthesis of the generative cell, which resulted in c. 40% of pollen grains arrested at the two-nucleate stage. The extended S phase did not impact the cell fate of the generative cell as shown by cell-specific markers. BICE1 encodes a plant homolog of human D123 protein that is required for G1 progression, but the underlying mechanism is unknown. Here we showed that BICE1 interacts with MCM4 and MCM7 of the pre-replication complex. Consistently, double mutations in BICE1 and MCM4, or MCM7, also led to bicellular pollen and condensed chromosomes. These suggest that BICE1 plays a role in modulating DNA replication via interaction with MCM4 and MCM7.

Identifiants

pubmed: 30484867
doi: 10.1111/nph.15610
doi:

Substances chimiques

AT4G05440 protein, Arabidopsis 0
Arabidopsis Proteins 0
Cell Cycle Proteins 0
DNA, Plant 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

588-603

Subventions

Organisme : National Natural Science Foundation of China
ID : 31330053
Pays : International
Organisme : National Natural Science Foundation of China
ID : 31570320
Pays : International

Informations de copyright

© 2018 Institute of Genetics and Developmental Biology, China New Phytologist © 2018 New Phytologist Trust.

Auteurs

Yan-Ping Long (YP)

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, East Lincui Road, Beijing, 100101, China.
College of Advanced Agricultural Sciences, University of the Chinese Academy of Sciences, Yuquan Road, Beijing, 100049, China.

Dong-Jiang Xie (DJ)

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, East Lincui Road, Beijing, 100101, China.
College of Advanced Agricultural Sciences, University of the Chinese Academy of Sciences, Yuquan Road, Beijing, 100049, China.

Yan-Yan Zhao (YY)

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, East Lincui Road, Beijing, 100101, China.

Dong-Qiao Shi (DQ)

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, East Lincui Road, Beijing, 100101, China.
College of Advanced Agricultural Sciences, University of the Chinese Academy of Sciences, Yuquan Road, Beijing, 100049, China.

Wei-Cai Yang (WC)

State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, East Lincui Road, Beijing, 100101, China.
College of Advanced Agricultural Sciences, University of the Chinese Academy of Sciences, Yuquan Road, Beijing, 100049, China.

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Classifications MeSH