Binding of β-carotene to whey proteins: Multi-spectroscopic techniques and docking studies.

Binding Sites Dose-Response Relationship, Drug Hydrophobic and Hydrophilic Interactions Molecular Docking Simulation Osmolar Concentration Protein Binding / drug effects Protein Domains Sodium Chloride / pharmacology Spectrum Analysis Temperature Whey Proteins / chemistry beta Carotene / metabolism
Circular dichroism Docking study Fluorescence quenching Thermodynamic parameters Whey protein isolate β-Carotene

Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
30 Mar 2019
Historique:
received: 16 05 2018
revised: 10 10 2018
accepted: 10 10 2018
entrez: 4 12 2018
pubmed: 7 12 2018
medline: 19 1 2019
Statut: ppublish

Résumé

The objective of this work was to study molecular binding between β-carotene (β-C) and whey protein isolate (WPI) as a function of pH (4-9), temperature (15, 25, and 35 °C), and NaCl concentration (0-0.25 M) using spectroscopic techniques and docking studies. The fluorescence quenching data showed that binding affinity increased with pH, temperature and ionic strength. The binding was entropy driven and involved mostly hydrophobic interactions. Three major whey proteins including β-lactoglobulin (β-Lg), α-lactalbumin (α-Lac), and bovine serum albumin (BSA) were bound to β-C with overall binding constant values of 1.31 × 10

Identifiants

DOI: 10.1016/j.foodchem.2018.10.057 PMID: 30502216
pubmed: 30502216
pii: S0308-8146(18)31822-3
doi: 10.1016/j.foodchem.2018.10.057
pii:
doi:

Substances chimiques

Whey Proteins 0
beta Carotene 01YAE03M7J
Sodium Chloride 451W47IQ8X

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

96-106

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

Zahra Allahdad (Z)

Department of Food Science and Technology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. Electronic address: zahra.allahdad@mail.um.ac.ir.

Mehdi Varidi (M)

Department of Food Science and Technology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. Electronic address: m.varidi@um.ac.ir.

Reza Zadmard (R)

Chemistry and Chemical Engineering Research Center of Iran, Tehran, Iran. Electronic address: zadmard@ccerci.ac.ir.

Ali Akbar Saboury (AA)

Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. Electronic address: saboury@ut.ac.ir.

Thomas Haertlé (T)

Poznań University of Life Sciences, Department of Animal Nutrition and Feed Management, ul. Wołyńska 33, 60-637 Poznań, Poland; UR 1268 Biopolymères Interactions Assemblages, INRA, 44316 Nantes Cedex 03, France. Electronic address: tom@haertle.fr.

Articles similaires

Exploring structural diversity across the protein universe with The Encyclopedia of Domains.

Andy M Lau, Nicola Bordin, Shaun M Kandathil et al.
1.00
Databases, Protein Protein Domains Protein Folding Proteins Deep Learning

Decoding the genomic terrain: functional insights into 14 chemosensory proteins in whitefly Bemisia tabaci Asia II-1.

M N Rudra Gouda, Sabtharishi Subramanian
1.00
Animals Hemiptera Insect Proteins Phylogeny Insecticides

Membrane potential stimulates ADP import and ATP export by the mitochondrial ADP/ATP carrier due to its positively charged binding site.

Vasiliki Mavridou, Martin S King, Andre Bazzone et al.
1.00
Adenosine Triphosphate Adenosine Diphosphate Mitochondrial ADP, ATP Translocases Binding Sites Mitochondria

A cytoplasmic osmosensing mechanism mediated by molecular crowding-sensitive DCP5.

Zhenyu Wang, Qiuhua Yang, Dan Zhang et al.
1.00
Arabidopsis Arabidopsis Proteins Osmotic Pressure Cytoplasm RNA, Messenger

Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes toxicologiques › Relation dose-effet des médicaments › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Phénomènes chimiques › Interactions hydrophobes et hydrophiles › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de docking moléculaire › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Phénomènes chimiques › Concentration osmolaire › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Produits chimiques inorganiques › Composés du sodium › Chlorure de sodium › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Aliments et boissons › Aliments › Protéines alimentaires › Protéines alimentaires animales › Protéines de lait › Protéines de lactosérum › Binding of β-carotene to whey proteins:...
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Caroténoïdes › Bêtacarotène › Binding of β-carotene to whey proteins:...