HSV susceptibility to acyclovir - genotypic and phenotypic characterization.
Acyclovir
/ pharmacology
Adult
Antiviral Agents
/ pharmacology
Drug Resistance, Viral
Female
Genes, Viral
Genotype
Herpes Simplex
/ diagnosis
Herpesvirus 1, Human
/ drug effects
Herpesvirus 2, Human
/ drug effects
Humans
Male
Microbial Sensitivity Tests
Mutation
Phenotype
Retrospective Studies
Sequence Analysis, DNA
Young Adult
Journal
Antiviral therapy
ISSN: 2040-2058
Titre abrégé: Antivir Ther
Pays: England
ID NLM: 9815705
Informations de publication
Date de publication:
2019
2019
Historique:
accepted:
14
11
2018
pubmed:
7
12
2018
medline:
12
5
2020
entrez:
4
12
2018
Statut:
ppublish
Résumé
Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples. A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique. Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay. These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.
Sections du résumé
BACKGROUND
Infections due to drug-resistant herpes simplex viruses (HSV) represent an important clinical concern, especially in immunocompromised patients. The present study was aimed at detecting acyclovir (ACV) susceptibility in HSV clinical samples.
METHODS
A total of 13 HSV-positive clinical samples (5 HSV-1 and 8 HSV-2) recovered from patients (1 immunocompromised and 12 of unknown immune status) were included in the study. The genotypic analysis involved an initial UL23 (thymidine kinase) gene sequencing, followed by a confirmatory phenotypic assay using plaque reduction technique.
RESULTS
Two novel amino acid changes, A37V and H283N, were detected in HSV-1 positive clinical samples, which were found to be susceptible to acyclovir (half maximal effective concentration = 1.5 µM) by plaque reduction assay.
CONCLUSIONS
These two novel amino acid changes could be therefore considered as natural polymorphisms, a phenomenon widely associated with the HSV-UL23 gene.
Substances chimiques
Antiviral Agents
0
Acyclovir
X4HES1O11F
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM