Sustained and regulated gene expression by Tet-inducible "all-in-one" retroviral vectors containing the HNRPA2B1-CBX3 UCOE
Animals
Cell Differentiation
/ drug effects
Cell Line, Tumor
Chromatin
/ metabolism
Chromosomal Proteins, Non-Histone
/ metabolism
Cytokines
/ metabolism
Doxycycline
/ pharmacology
Gene Expression
Genetic Vectors
/ metabolism
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
/ metabolism
Humans
Induced Pluripotent Stem Cells
/ cytology
Lentivirus
/ genetics
Mice
Phosphoglycerate Kinase
/ metabolism
Promoter Regions, Genetic
Receptors, Thrombopoietin
/ metabolism
Tetracycline
/ pharmacology
Transcriptional Activation
/ genetics
Transgenes
A2UCOE
Retroviral vector
Tet-inducible
iPS cells
Journal
Biomaterials
ISSN: 1878-5905
Titre abrégé: Biomaterials
Pays: Netherlands
ID NLM: 8100316
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
09
09
2018
revised:
01
11
2018
accepted:
06
11
2018
pubmed:
6
12
2018
medline:
7
3
2020
entrez:
4
12
2018
Statut:
ppublish
Résumé
Genetic modification of induced pluripotent stem (iPS) cells may be necessary for the generation of effector cells for cellular therapies. Hereby, it can be important to induce transgene expression at restricted and defined time windows, especially if it interferes with pluripotency or differentiation. To achieve this, inducible expression systems can be used such as the tetracycline-inducible retroviral vector system, however, retroviral expression can be subjected to epigenetic silencing or to position-effect variegation. One strategy to overcome this is the incorporation of ubiquitous chromatin opening elements (UCOE
Identifiants
pubmed: 30508767
pii: S0142-9612(18)30783-X
doi: 10.1016/j.biomaterials.2018.11.006
pii:
doi:
Substances chimiques
Chromatin
0
Chromosomal Proteins, Non-Histone
0
Cytokines
0
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
0
Receptors, Thrombopoietin
0
Phosphoglycerate Kinase
EC 2.7.2.3
Tetracycline
F8VB5M810T
Doxycycline
N12000U13O
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
486-499Informations de copyright
Copyright © 2018. Published by Elsevier Ltd.