Silver nanoparticle modulates the aggregation of beta-lactoglobulin and induces to form rod-like aggregates.
Amyloid fibrillar aggregates
Docking
Rod-shaped aggregates
Scanning electron microscopy (SEM)
Silver nanoparticle (SNP)
Transmission electron microscopy (TEM)
β-Lactoglobulin (β-lg)
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Mar 2019
15 Mar 2019
Historique:
received:
10
09
2018
revised:
23
11
2018
accepted:
02
12
2018
pubmed:
12
12
2018
medline:
17
5
2019
entrez:
12
12
2018
Statut:
ppublish
Résumé
Silver nanoparticles (SNPs) have been increasingly used in medicines and biomaterials as a drug carriers and diagnostic or therapeutic material due to their smaller size, large surface area and cell penetration ability. Here we report the preparation of SNPs of diameter 10 ± 3 nm by using silver nitrate and sodium borohydride and the interaction of synthesized SNPs with our model protein β-lactoglobulin (β-lg) in 10 mM phosphate buffer at pH 7.5 after thermal exposure at 75 °C. Heat exposed β-lg forms amyloidal fibrillar aggregates whereas this protein aggregates adopt rod-like shape instead of fibrillar structure in presence of SNP under the same conditions. Size of the synthesized SNPs is confirmed by UV-Visible spectroscopy, SEM and TEM. Interactions and subsequent formation of molecular assembly of heat stressed β-lg with SNP were investigated using Th-T assay and ANS binding assay, DLS, RLS, CD, FT-IR, SEM, TEM. Docking study parallely also support the experimental findings.
Identifiants
pubmed: 30528992
pii: S0141-8130(18)34835-9
doi: 10.1016/j.ijbiomac.2018.12.039
pii:
doi:
Substances chimiques
Amyloid
0
Lactoglobulins
0
Protein Aggregates
0
Silver
3M4G523W1G
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
596-604Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.