Fasciola hepatica and Fasciola hybrid form co-existence in yak from Tibet of China: application of rDNA internal transcribed spacer.
Animals
Cattle
Tibet
Fasciola
/ genetics
Fascioliasis
/ veterinary
DNA, Ribosomal Spacer
/ genetics
Cattle Diseases
/ parasitology
DNA, Helminth
/ genetics
Fasciola hepatica
/ genetics
Sequence Analysis, DNA
Polymorphism, Single Nucleotide
Hybridization, Genetic
High-Throughput Nucleotide Sequencing
Fasciola
Nuclear rDNA internal transcribed spacer
Single-nucleotide polymorphisms
Tibet
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
01 Nov 2024
01 Nov 2024
Historique:
received:
08
08
2024
accepted:
16
10
2024
medline:
1
11
2024
pubmed:
1
11
2024
entrez:
1
11
2024
Statut:
epublish
Résumé
Fasciolosis is a parasitic disease affecting humans and livestock, caused by digenean trematodes of the genus Fasciola, primarily F. hepatica and F. gigantica. This study investigates the coexistence of these species and their hybrids in yaks from Tibet, China. We analyzed the nuclear rDNA internal transcribed spacer (ITS) regions, including ITS1 and ITS2, through Sanger sequencing and Next-Generation Sequencing (NGS) to assess single-nucleotide polymorphisms (SNPs). Our results reveal that one specimen (NM008B) is identical to pure F. hepatica, while another (NM008A) contains genetic information from both F. hepatica and F. gigantica, indicating potential hybridization or introgression. The morphological analysis reveals that the collected adult F. hepatica specimens exhibit distinct characteristics, while the hybrid specimens display "intermediate" features of F. hepatica and F. gigantica. This study is the first to document the coexistence of F. hepatica and hybrid Fasciola forms in a single yak. The findings underscore the complexities of hybridization dynamics and the necessity for advanced molecular techniques in accurately identifying Fasciola species. Future research should focus on mitochondrial DNA and other nuclear gene analysis to further elucidate the nature of these hybrids and their ecological implications.
Identifiants
pubmed: 39482528
doi: 10.1007/s00436-024-08383-y
pii: 10.1007/s00436-024-08383-y
doi:
Substances chimiques
DNA, Ribosomal Spacer
0
DNA, Helminth
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
366Subventions
Organisme : The State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement
ID : XZNKY-CZ-2022-016-08
Organisme : The State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement
ID : XZNKY-CZ-2022-016-08
Organisme : Base and Talent Program of Science and Technology Plan in Tibet Autonomous Region
ID : XZ202401JD0012
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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