Genome editing in plants by engineered CRISPR-Cas9 recognizing NG PAM.
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
07
03
2018
accepted:
07
11
2018
pubmed:
12
12
2018
medline:
14
6
2019
entrez:
12
12
2018
Statut:
ppublish
Résumé
Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1) can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.
Identifiants
pubmed: 30531939
doi: 10.1038/s41477-018-0321-8
pii: 10.1038/s41477-018-0321-8
doi:
Substances chimiques
RNA, Guide
0
CRISPR-Associated Protein 9
EC 3.1.-
Cas9 endonuclease Streptococcus pyogenes
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14-17Références
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