Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis.
ACPA
ELISA
Epitope mapping
Histone H4
Peptide antigen
Rheumatoid arthritis
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
13
12
2018
pubmed:
13
12
2018
medline:
7
6
2019
Statut:
ppublish
Résumé
Deiminated proteins are the target of diagnostic anti-citrullinated peptide/protein autoantibodies (ACPA) in rheumatoid arthritis (RA). Deiminated histone H4 contained in the neutrophil extracellular traps reacts with ACPA, becoming an interesting diagnostic antigen for RA. The identification of the ACPA binding site in histone H4 was performed experimentally by mapping the complete sequence. The method describes the synthesis of an overlapping peptide library covering the entire deiminated sequence of H4 and its further evaluation in ELISA. A detailed description of an ELISA protocol to test RA patients' sera against the synthesized peptides and ACPA is provided.
Identifiants
pubmed: 30539581
doi: 10.1007/978-1-4939-8949-2_18
doi:
Substances chimiques
Autoantibodies
0
Histones
0
Immunoglobulin G
0
Peptide Library
0
Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM