Epidemiological investigation of an Acinetobacter baumannii outbreak using core genome multilocus sequence typing.
Acinetobacter Infections
/ epidemiology
Acinetobacter baumannii
/ drug effects
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ genetics
Bacterial Typing Techniques
/ methods
Carbapenem-Resistant Enterobacteriaceae
/ genetics
Carbapenems
Disease Outbreaks
Humans
Intensive Care Units
Italy
Microbial Sensitivity Tests
Molecular Epidemiology
Multilocus Sequence Typing
/ methods
Phylogeny
Polymerase Chain Reaction
Whole Genome Sequencing
beta-Lactamases
/ genetics
Acinetobacter baumannii
Carbapenem resistance
Core genome MLST
Epidemiology
Journal
Journal of global antimicrobial resistance
ISSN: 2213-7173
Titre abrégé: J Glob Antimicrob Resist
Pays: Netherlands
ID NLM: 101622459
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
16
07
2018
revised:
28
11
2018
accepted:
29
11
2018
pubmed:
17
12
2018
medline:
30
5
2020
entrez:
17
12
2018
Statut:
ppublish
Résumé
Carbapenem-resistant Acinetobacter baumannii (CRAB) is a serious nosocomial pathogen that causes a variety of serious, often life-threatening, infections and outbreaks. This study aimed to investigate the molecular epidemiology of clinical CRAB isolates from an outbreak that occurred in the intensive care unit (ICU) of an Italian hospital. From December 2016 to April 2017, 13 CRAB isolates were collected from seven patients treated in the ICU at 'L. Spallanzani' Hospital (Rome, Italy). Typing was performed by repetitive extragenic palindromic PCR (rep-PCR) using a DiversiLab All isolates showed a carbapenem-resistant profile and carried the bla These results show that WGS by cgMLST is a valuable tool, better suited for prompt epidemiological investigations than traditional typing methods because of its higher discriminatory ability in determining clonal relatedness.
Identifiants
pubmed: 30553929
pii: S2213-7165(18)30244-3
doi: 10.1016/j.jgar.2018.11.027
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Carbapenems
0
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
245-249Informations de copyright
Copyright © 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.