Melanoma-related changes in skin microbiome.
Journal
Folia microbiologica
ISSN: 1874-9356
Titre abrégé: Folia Microbiol (Praha)
Pays: United States
ID NLM: 0376757
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
24
04
2018
accepted:
30
11
2018
pubmed:
17
12
2018
medline:
19
9
2019
entrez:
17
12
2018
Statut:
ppublish
Résumé
Melanoma is the least common form of skin tumor, but it is potentially the most dangerous and responsible for the majority of skin cancer deaths. We suggest that the skin microbiome might be changed during the progression of melanoma. The aim of this study is to compare the composition of the skin microbiota between different locations (skin and melanoma) of a MeLiM (Melanoma-bearing Libechov Minipig) pig model (exophytic melanoma). Ninety samples were used for PCR-DGGE analysis with primers specifically targeting the V3 region of the 16S rRNA gene. The profiles were used for cluster analysis by UPGMA and principal coordinate analysis PCoA and also to calculate the diversity index (Simpson index of diversity). By comparing the obtained results, we found that both bacterial composition and diversity were significantly different between the skin and melanoma microbiomes. The abundances of Fusobacterium and Trueperella genera were significantly increased in melanoma samples, suggesting a strong relationship between melanoma development and skin microbiome changes.
Identifiants
pubmed: 30554379
doi: 10.1007/s12223-018-00670-3
pii: 10.1007/s12223-018-00670-3
doi:
Substances chimiques
DNA Primers
0
DNA, Bacterial
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
435-442Subventions
Organisme : Fakultu Agrobiologie, Potravinových a Prírodních Zdroju, Ceská Zemedelská Univerzita v Praze
ID : CIGA 20162019
Organisme : Fakultu Agrobiologie, Potravinových a Prírodních Zdroju, Ceská Zemedelská Univerzita v Praze
ID : CIGA 20162001
Organisme : Ministerstvo Školství, Mládeže a Telovýchovy
ID : CZ.02.1.01/0.0/0.0/15_003/0000460
Organisme : Ministerstvo Školství, Mládeže a Telovýchovy
ID : LO1609
Références
Dermatol Surg. 2000 Feb;26(2):127-9
pubmed: 10691940
Br J Biomed Sci. 2007;64(2):74-7
pubmed: 17633142
Nature. 2007 Oct 18;449(7164):811-8
pubmed: 17943117
Science. 2009 May 29;324(5931):1190-2
pubmed: 19478181
Clin Infect Dis. 2009 Sep 1;49(5):766-72
pubmed: 19635028
J Clin Oncol. 2009 Dec 20;27(36):6199-206
pubmed: 19917835
Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14799-804
pubmed: 20668241
J Clin Microbiol. 2011 Dec;49(12):4377-8
pubmed: 21998429
Genome Res. 2012 Feb;22(2):299-306
pubmed: 22009989
Genome Res. 2012 Feb;22(2):292-8
pubmed: 22009990
Science. 2012 Aug 31;337(6098):1115-9
pubmed: 22837383
Nat Commun. 2013;4:1431
pubmed: 23385576
Vet Microbiol. 2013 May 3;163(3-4):344-50
pubmed: 23415031
J Innate Immun. 2014;6(3):253-62
pubmed: 23796786
Can Vet J. 2013 Feb;54(2):139-44
pubmed: 23904636
Semin Immunol. 2013 Nov 30;25(5):370-7
pubmed: 24268438
Microb Ecol. 2014 Apr;67(3):679-89
pubmed: 24402361
J Vet Med Sci. 2014 May;76(5):715-8
pubmed: 24419876
PLoS One. 2014 Jan 08;9(1):e83197
pubmed: 24421875
Eur J Clin Microbiol Infect Dis. 2014 Aug;33(8):1381-90
pubmed: 24599709
ISME J. 2014 Nov;8(11):2207-17
pubmed: 24858782
Pol J Vet Sci. 2014;17(2):395-404
pubmed: 24988872
An Bras Dermatol. 2014 Jul-Aug;89(4):666-8
pubmed: 25054761
Immunity. 2015 Feb 17;42(2):344-355
pubmed: 25680274
Microb Ecol. 2015 Aug;70(2):534-44
pubmed: 25704317
Oncotarget. 2015 Mar 30;6(9):7209-20
pubmed: 25797243
Oncotarget. 2015 Sep 8;6(26):22613-23
pubmed: 26158901
Eur J Dermatol. 2016 Jan-Feb;26(1):49-55
pubmed: 26680010
Cell J. 2016 Winter;17(4):601-7
pubmed: 26862519
PLoS One. 2016 Nov 8;11(11):e0166176
pubmed: 27824921
Gastroenterology. 2017 Mar;152(4):694-696
pubmed: 28143770
Nat Rev Cancer. 2017 May;17(5):271-285
pubmed: 28303904
J Cancer Res Ther. 2017 Apr-Jun;13(2):246-251
pubmed: 28643742
Int J Mol Cell Med. 2017 Spring;6(2):66-76
pubmed: 28890883
Front Microbiol. 2017 Oct 06;8:1929
pubmed: 29056930
Exp Ther Med. 2017 Nov;14(5):4586-4594
pubmed: 29104666
Bioimpacts. 2017;7(3):193-198
pubmed: 29159146
Front Immunol. 2017 Nov 17;8:1553
pubmed: 29209314
Breast Care (Basel). 2017 Oct;12(5):335-339
pubmed: 29234255
Immunotherapy. 2018 Feb;10(2):119-129
pubmed: 29260626
Nat Rev Microbiol. 2018 Mar;16(3):143-155
pubmed: 29332945
Sci Adv. 2018 Feb 28;4(2):eaao4502
pubmed: 29507878
Cancer Manag Res. 2018 May 16;10:1219-1229
pubmed: 29844701
Cancer. 1973 Dec;32(6):1446-57
pubmed: 4757934
Appl Environ Microbiol. 1993 Mar;59(3):695-700
pubmed: 7683183
Methods Enzymol. 1996;266:131-41
pubmed: 8743682