Highly Efficient Protein-free Membrane Fusion: A Giant Vesicle Study.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
08 01 2019
08 01 2019
Historique:
received:
20
08
2018
revised:
14
11
2018
accepted:
19
11
2018
pubmed:
24
12
2018
medline:
3
1
2020
entrez:
24
12
2018
Statut:
ppublish
Résumé
Membrane fusion is a ubiquitous process in biology and is a prerequisite for many intracellular delivery protocols relying on the use of liposomes as drug carriers. Here, we investigate in detail the process of membrane fusion and the role of opposite charges in a protein-free lipid system based on cationic liposomes (LUVs, large unilamellar vesicles) and anionic giant unilamellar vesicles (GUVs) composed of different palmitoyloleoylphosphatidylcholine (POPC)/palmitoyloleoylphosphatidylglycerol (POPG) molar ratios. By using a set of optical-microscopy- and microfluidics-based methods, we show that liposomes strongly dock to GUVs of pure POPC or low POPG fraction (up to 10 mol%) in a process mainly associated with hemifusion and membrane tension increase, commonly leading to GUV rupture. On the other hand, docked LUVs quickly and very efficiently fuse with negative GUVs of POPG fractions at or above 20 mol%, resulting in dramatic GUV area increase in a charge-dependent manner; the vesicle area increase is deduced from GUV electrodeformation. Importantly, both hemifusion and full fusion are leakage-free. Fusion efficiency is quantified by the lipid transfer from liposomes to GUVs using fluorescence resonance energy transfer (FRET), which leads to consistent results when compared to fluorescence-lifetime-based FRET. We develop an approach to deduce the final composition of single GUVs after fusion based on the FRET efficiency. The results suggest that fusion is driven by membrane charge and appears to proceed up to charge neutralization of the acceptor GUV.
Identifiants
pubmed: 30579564
pii: S0006-3495(18)34450-3
doi: 10.1016/j.bpj.2018.11.3128
pmc: PMC6342729
pii:
doi:
Substances chimiques
Phosphatidylcholines
0
Phosphatidylglycerols
0
Unilamellar Liposomes
0
1-palmitoyl-2-oleoylglycero-3-phosphoglycerol
81490-05-3
1-palmitoyl-2-oleoylphosphatidylcholine
TE895536Y5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
79-91Informations de copyright
Copyright © 2018 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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