Biophysical Parameters of the Sec14 Phospholipid Exchange Cycle.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
08 01 2019
08 01 2019
Historique:
received:
21
09
2018
revised:
24
10
2018
accepted:
28
11
2018
pubmed:
26
12
2018
medline:
3
1
2020
entrez:
25
12
2018
Statut:
ppublish
Résumé
Sec14, the major yeast phosphatidylcholine (PC)/phosphatidylinositol (PI) transfer protein (PITP), coordinates PC and PI metabolism to facilitate an appropriate and essential lipid signaling environment for membrane trafficking from trans-Golgi membranes. The Sec14 PI/PC exchange cycle is essential for its essential biological activity, but fundamental aspects of how this PITP executes its lipid transfer cycle remain unknown. To address some of these outstanding issues, we applied time-resolved small-angle neutron scattering for the determination of protein-mediated intervesicular movement of deuterated and hydrogenated phospholipids in vitro. Quantitative analysis by small-angle neutron scattering revealed that Sec14 PI- and PC-exchange activities were sensitive to both the lipid composition and curvature of membranes. Moreover, we report that these two parameters regulate lipid exchange activity via distinct mechanisms. Increased membrane curvature promoted both membrane binding and lipid exchange properties of Sec14, indicating that this PITP preferentially acts on the membrane site with a convexly curved face. This biophysical property likely constitutes part of a mechanism by which spatial specificity of Sec14 function is determined in cells. Finally, wild-type Sec14, but not a mixture of Sec14 proteins specifically deficient in either PC- or PI-binding activity, was able to effect a net transfer of PI or PC down opposing concentration gradients in vitro.
Identifiants
pubmed: 30580923
pii: S0006-3495(18)34453-9
doi: 10.1016/j.bpj.2018.11.3131
pmc: PMC6342728
pii:
doi:
Substances chimiques
Phosphatidylcholines
0
Phosphatidylinositols
0
Phospholipid Transfer Proteins
0
SEC14 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
Unilamellar Liposomes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
92-103Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM044530
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131804
Pays : United States
Informations de copyright
Copyright © 2018 Biophysical Society. All rights reserved.
Références
J Phys Chem B. 2009 May 14;113(19):6745-8
pubmed: 19385639
Dev Cell. 2018 Feb 5;44(3):378-391.e5
pubmed: 29396115
Mol Cell. 2008 Feb 1;29(2):191-206
pubmed: 18243114
Trends Biochem Sci. 2010 Mar;35(3):150-60
pubmed: 19926291
Nat Commun. 2015 Apr 07;6:6671
pubmed: 25849868
Traffic. 2015 Jan;16(1):19-34
pubmed: 25262571
Electrophoresis. 2010 Oct;31(21):3573-9
pubmed: 20967768
Chem Phys Lipids. 2006 Jun;141(1-2):169-78
pubmed: 16584720
Nature. 2014 Jun 5;510(7503):48-57
pubmed: 24899304
Nat Commun. 2014 Sep 15;5:4916
pubmed: 25222832
J Cell Biol. 2002 Apr 1;157(1):63-77
pubmed: 11916983
Mol Biol Cell. 2007 May;18(5):1928-42
pubmed: 17344474
Nature. 1990 Oct 11;347(6293):561-2
pubmed: 2215682
Cell. 2012 Feb 17;148(4):702-15
pubmed: 22341443
Phys Rev Lett. 2007 Jun 8;98(23):238101
pubmed: 17677937
Clin Lipidol. 2010 Dec 1;5(6):867-897
pubmed: 21603057
J Cell Biol. 1999 Aug 23;146(4):741-54
pubmed: 10459010
Nat Chem Biol. 2014 Jan;10(1):76-84
pubmed: 24292071
Eur Biophys J. 2006 Feb;35(3):181-9
pubmed: 16283292
EMBO J. 1996 Dec 2;15(23):6447-59
pubmed: 8978672
Nat Rev Mol Cell Biol. 2008 Feb;9(2):112-24
pubmed: 18216768
EMBO J. 2018 May 2;37(9):
pubmed: 29599178
Biochemistry. 1985 May 21;24(11):2773-81
pubmed: 4027225
Mol Biol Cell. 2000 Jun;11(6):1989-2005
pubmed: 10848624
Biophys J. 2007 Dec 1;93(11):3900-6
pubmed: 17704174
J Mol Biol. 2007 Feb 16;366(2):551-62
pubmed: 17187824
Science. 2013 May 31;340(6136):1106-10
pubmed: 23599266
Proteins. 2005 Nov 1;61(2):412-22
pubmed: 16121400
PLoS One. 2014 Jul 01;9(7):e101550
pubmed: 24983950
Nature. 1998 Jan 29;391(6666):506-10
pubmed: 9461221
Trends Cell Biol. 2011 Feb;21(2):113-21
pubmed: 21282087
Mol Biol Cell. 2009 Feb;20(3):780-90
pubmed: 19037109
Cell Logist. 2012 Jul 1;2(3):151-160
pubmed: 23181198
J Biol Chem. 1959 Mar;234(3):466-8
pubmed: 13641241
J Biol Chem. 1990 Mar 15;265(8):4711-7
pubmed: 2407740
Biochim Biophys Acta. 1989 Nov 27;986(2):301-9
pubmed: 2686754
Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22245-50
pubmed: 20080790
Crit Rev Biochem Mol Biol. 2006 Jan-Feb;41(1):21-49
pubmed: 16455519
Langmuir. 2016 Dec 27;32(51):13697-13705
pubmed: 27936747
FASEB J. 2013 Dec;27(12):5131-40
pubmed: 24018064
J Cell Biol. 2009 May 18;185(4):601-12
pubmed: 19433450