Recombinant rice xylanase-inhibiting protein inhibits GH11 endo-xylanases through competitive inhibition.
Competitive inhibition
Fluorescence spectrum
Hydrolysis
Rice xylanase-inhibiting protein (riceXIP)
Xylanase
Journal
Protein expression and purification
ISSN: 1096-0279
Titre abrégé: Protein Expr Purif
Pays: United States
ID NLM: 9101496
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
22
06
2018
revised:
23
11
2018
accepted:
28
12
2018
pubmed:
2
1
2019
medline:
9
1
2020
entrez:
2
1
2019
Statut:
ppublish
Résumé
The ricexip gene, which encodes the rice xylanase-inhibiting protein (riceXIP), was recombinantly expressed in Pichia pastoris GS115 under the control of AOX1 promoter. Recombinant riceXIP (rePriceXIP) was secreted into the supernatant and purified to homogeneity with the use of Ni-affinity resin. The molecular mass of rePriceXIP was approximately 44.0 kDa. RePriceXIP significantly inhibited the activity of GH11 xylanases in a concentration-dependent manner. The optimal inhibitory activity of rePriceXIP on GH11 xylanases (TfxA_CD214, reBaxA454, and TfxA_CD526) occurred at 40 °C for 30 min. The IC
Identifiants
pubmed: 30599198
pii: S1046-5928(18)30354-1
doi: 10.1016/j.pep.2018.12.008
pii:
doi:
Substances chimiques
Enzyme Inhibitors
0
Recombinant Proteins
0
Aldehyde Oxidase
EC 1.2.3.1
Endo-1,4-beta Xylanases
EC 3.2.1.8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17-24Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.