Reliable method for the detection of horseradish peroxidase activity and enzyme kinetics.

Enzyme-catalyzed reactions are complicated and their kinetics depend on various chemical and physical factors. In a simple enzyme-catalyzed reaction, the enzyme kinetics often involve two or more substrates. However, this complexity is often ignored when studying enzyme kinetics or determining enzyme activity. Such an example is horseradish peroxidase (HRP), whose activity and kinetics in the reduction of H