Role of TRF2 and TPP1 regulation in idiopathic recurrent pregnancy loss.
Abortion, Habitual
/ metabolism
Adult
Aminopeptidases
/ biosynthesis
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
/ biosynthesis
Down-Regulation
Female
Fetus
/ metabolism
Gene Expression Regulation, Developmental
Humans
Pregnancy
Shelterin Complex
Telomere Homeostasis
Telomere-Binding Proteins
/ biosynthesis
Telomeric Repeat Binding Protein 2
/ biosynthesis
Idiopathic recurrent pregnancy loss
Shelterin proteins TRF1
TPP1
TRF2
Universal STELA
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Apr 2019
15 Apr 2019
Historique:
received:
27
07
2018
revised:
31
12
2018
accepted:
03
01
2019
pubmed:
7
1
2019
medline:
20
6
2019
entrez:
7
1
2019
Statut:
ppublish
Résumé
Telomeres are the tandem repeats (TTAGGG) present at the ends of the chromosomes that ensure chromosome stability and protect chromosomes from degradation. Telomeres in somatic human cells shorten after every cellular division and are linked to the cellular senescence. In this study we have investigated telomere length and expression of shelterin genes in aborted fetus material from idiopathic recurrent pregnancy losses. Telomere length was measured using Telomere Restriction Fragment Length (TRF) analysis. The gene expression levels for important shelterin complex proteins (TRF1, TRF2, POT1, and TPP1) were determined by Real-time Quantitative Reverse Transcriptase PCR (qRT-PCR). Our results demonstrated down regulation of TRF2 and TPP1 and a strong decline in average telomere length in abort material from women suffering from idiopathic recurrent pregnancy loss. We suggest that shorter telomere length and downregulation of the major shelterin components TRF2 and TPP1 leading to "telomere uncapping", might play a critical role in recurrent pregnancy loss.
Identifiants
pubmed: 30611803
pii: S0141-8130(18)33879-0
doi: 10.1016/j.ijbiomac.2019.01.011
pii:
doi:
Substances chimiques
ACD protein, human
0
Shelterin Complex
0
TERF2 protein, human
0
Telomere-Binding Proteins
0
Telomeric Repeat Binding Protein 2
0
Aminopeptidases
EC 3.4.11.-
Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
EC 3.4.14.-
Types de publication
Clinical Trial
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
306-310Informations de copyright
Copyright © 2019. Published by Elsevier B.V.