Fibroblast-derived integration-free iPSC line ISRM-NBS1 from an 18-year-old Nijmegen Breakage Syndrome patient carrying the homozygous NBN c.657_661del5 mutation.
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
13
11
2018
accepted:
17
12
2018
pubmed:
8
1
2019
medline:
7
8
2019
entrez:
8
1
2019
Statut:
ppublish
Résumé
Human fibroblasts cells from a female diagnosed with Nijmegen Breakage Syndrome (NBS) carrying the homozygous NBN c.657_661del5 mutation were used to generate integration-free induced pluripotent stem cells (iPSCs) by over-expressing episomal-based plasmids harbouring OCT4, SOX2, NANOG, KLF4, c-MYC and LIN28. The derived iPSC line - ISRM-NBS1 was defined as pluripotent based on (i) expression of pluripotency-associated markers (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptome of the iPSC line and the human embryonic stem cell line H1 with a Pearson correlation of 0.955.
Identifiants
pubmed: 30616142
pii: S1873-5061(18)30307-6
doi: 10.1016/j.scr.2018.101372
pii:
doi:
Substances chimiques
Cell Cycle Proteins
0
KLF4 protein, human
0
Kruppel-Like Factor 4
0
NBN protein, human
0
Nuclear Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
101372Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.