Relationship between N,N-dimethylformamide exposure, PNPLA3, GCKR, COL13A1 and TM6SF2 genes, and liver injury.
Adaptor Proteins, Signal Transducing
/ genetics
Adult
Chemical and Drug Induced Liver Injury
/ etiology
China
Cohort Studies
Collagen Type XIII
/ genetics
Dimethylformamide
/ toxicity
Female
Gene-Environment Interaction
Humans
Lipase
/ genetics
Male
Membrane Proteins
/ genetics
Middle Aged
Occupational Exposure
/ adverse effects
Polymorphism, Single Nucleotide
Threshold Limit Values
COL13A1 gene
GCKR gene
Liver injury
N,N-dimethylformamide exposure
PNPLA3 gene
TM6SF2 gene
Journal
Ecotoxicology and environmental safety
ISSN: 1090-2414
Titre abrégé: Ecotoxicol Environ Saf
Pays: Netherlands
ID NLM: 7805381
Informations de publication
Date de publication:
30 Apr 2019
30 Apr 2019
Historique:
received:
12
10
2018
revised:
06
12
2018
accepted:
18
12
2018
pubmed:
8
1
2019
medline:
19
3
2019
entrez:
8
1
2019
Statut:
ppublish
Résumé
Current researches show that N,N-dimethylformamide (DMF) exposure is associated with liver injury, but it is debatable whether PNPLA3, GCKR, COL13A1 and TM6SF2 gene polymorphisms are associated with liver injury. Our objective was to examine the relationship among DMF exposure, PNPLA3 rs738409, GCKR rs780094, COL13A1 rs1227756, TM6SF2 rs58542926 and liver injury. The cohort consisted of 461 workers exposed above the DMF threshold limit value (TLV) and 211 exposed below the DMF TLV in China, who were followed for 5 years. The relationship between the measured dose of DMF and the relative risk (RR) of liver injury was also investigated by Poisson analysis. Logistic regression models were used to examine the association between measured dose of DMF, gene locus, and RR for liver injury. All workers had a annual physical examinations were conducted at certified physical examination centers in Taicang CDC, including liver serum transaminase assessment and abdominal ultrasound. Genomic DNA was extracted from peripheral blood leukocytes using a genomic DNA extraction kit. The incidence of liver injury in the above DMF TLV group was significantly higher than in the below DMF TLV group. GCKR rs780094 was associated with liver injury. The interaction among the GCKR rs780094, DMF exposure and liver injury showed no significant association. Our data indicated that in DMF exposure, GCKR rs780094 may contribute to the risk of liver injury. Our results suggest that GCKR rs780094 is a useful genetic marker to help identify liver injury.
Sections du résumé
BACKGROUND
BACKGROUND
Current researches show that N,N-dimethylformamide (DMF) exposure is associated with liver injury, but it is debatable whether PNPLA3, GCKR, COL13A1 and TM6SF2 gene polymorphisms are associated with liver injury. Our objective was to examine the relationship among DMF exposure, PNPLA3 rs738409, GCKR rs780094, COL13A1 rs1227756, TM6SF2 rs58542926 and liver injury.
METHODS
METHODS
The cohort consisted of 461 workers exposed above the DMF threshold limit value (TLV) and 211 exposed below the DMF TLV in China, who were followed for 5 years. The relationship between the measured dose of DMF and the relative risk (RR) of liver injury was also investigated by Poisson analysis. Logistic regression models were used to examine the association between measured dose of DMF, gene locus, and RR for liver injury. All workers had a annual physical examinations were conducted at certified physical examination centers in Taicang CDC, including liver serum transaminase assessment and abdominal ultrasound. Genomic DNA was extracted from peripheral blood leukocytes using a genomic DNA extraction kit.
RESULTS
RESULTS
The incidence of liver injury in the above DMF TLV group was significantly higher than in the below DMF TLV group. GCKR rs780094 was associated with liver injury. The interaction among the GCKR rs780094, DMF exposure and liver injury showed no significant association.
CONCLUSIONS
CONCLUSIONS
Our data indicated that in DMF exposure, GCKR rs780094 may contribute to the risk of liver injury. Our results suggest that GCKR rs780094 is a useful genetic marker to help identify liver injury.
Identifiants
pubmed: 30616151
pii: S0147-6513(18)31348-4
doi: 10.1016/j.ecoenv.2018.12.055
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
COL13A1 protein, human
0
Collagen Type XIII
0
GCKR protein, human
0
Membrane Proteins
0
TM6SF2 protein, human
0
Dimethylformamide
8696NH0Y2X
Lipase
EC 3.1.1.3
adiponutrin, human
EC 3.1.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
347-351Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.