Use of Lactobacillus crispatus to produce a probiotic cheese as potential gender food for preventing gynaecological infections.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
05
05
2018
accepted:
17
11
2018
entrez:
10
1
2019
pubmed:
10
1
2019
medline:
24
9
2019
Statut:
epublish
Résumé
This research is aimed to evaluate the suitability of Squacquerone cheese to support the viability of Lactobacillus crispatus BC4, a vaginal strain endowed with a strong antimicrobial activity against urogenital pathogens and foodborne microorganisms, in order to recommend a gender food for woman wellbeing. The viability of L. crispatus BC4, used as adjunct culture, was evaluated during the refrigerated storage of Squacquerone cheese, as well as when the cheese was subjected to simulated stomach-duodenum passage tested by the patented Simulator of the Human Intestinal Microbial Ecosystem (SHIME). Moreover, the effects of L. crispatus BC4 addition were evaluated on product hydrolytic patterns, in terms of proteolysis, lipolysis and volatile molecule profiles. The data showed that L. crispatus BC4 maintained high viability, also in presence of physiological stress conditions, until the end of the refrigerated storage. Moreover, the inclusion of L. crispatus BC4 gave rise to cheese product with higher score of overall acceptability when compared to control cheese. In addition, the survival of L. crispatus BC4, carried in test cheese, in gastro intestinal conditions was confirmed by SHIME. The results showed that the vaginal Lactobacillus strain was more affected by the low pH of the stomach, simulated by the SHIME reactor, rather than to bile salts and pancreatic juices. Although only in vivo trials will be able to confirm the functionality of the cheese in the vaginal environment, these data represent a first step towards the employment of the Squacquerone cheese as probiotic food able to promote the woman's health by preventing gynaecological infections.
Identifiants
pubmed: 30625157
doi: 10.1371/journal.pone.0208906
pii: PONE-D-18-13541
pmc: PMC6326422
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0208906Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Sci Rep. 2016 Jun 29;6:29024
pubmed: 27354249
Front Cell Infect Microbiol. 2017 Dec 06;7:502
pubmed: 29270390
Q Rev Biol. 2012 Dec;87(4):325-41
pubmed: 23397797
FEMS Microbiol Lett. 2012 Sep;334(1):1-15
pubmed: 22568660
Clin Nutr. 2018 Aug;37(4):1216-1223
pubmed: 28669666
Food Chem. 2015 May 15;175:121-7
pubmed: 25577060
Immunol Lett. 2013 Nov-Dec;156(1-2):102-9
pubmed: 24120511
J Dairy Sci. 2008 Feb;91(2):500-12
pubmed: 18218736
Appetite. 2006 Mar;46(2):152-63
pubmed: 16469413
Appl Microbiol Biotechnol. 1993 May;39(2):254-8
pubmed: 7763732
J Biomed Biotechnol. 2011;2011:981214
pubmed: 21772792
Pharmacol Res. 2014 Sep;87:160-5
pubmed: 24880136
J Gynecol Obstet Hum Reprod. 2018 Feb;47(2):81-86
pubmed: 29196153
Int J Food Microbiol. 2008 Dec 10;128(2):329-35
pubmed: 18973961
FEMS Immunol Med Microbiol. 2001 Feb;30(1):49-52
pubmed: 11172991
Crit Rev Food Sci Nutr. 2018 Jul 3;58(10):1735-1746
pubmed: 28071925
Clin Ther. 2008 Mar;30(3):453-68
pubmed: 18405785
Allergol Int. 2017 Oct;66(4):515-522
pubmed: 28826938
Am J Obstet Gynecol. 2011 Apr;204(4):305.e1-7
pubmed: 21272848
Eur J Obstet Gynecol Reprod Biol. 2013 May;168(1):75-9
pubmed: 23395559
Mol Aspects Med. 2011 Feb;32(1):1-70
pubmed: 21356234
Am J Clin Nutr. 2011 Dec;94(6 Suppl):2000S-2005S
pubmed: 21543533
Front Microbiol. 2015 Jul 09;6:687
pubmed: 26217319
Front Microbiol. 2017 Apr 04;8:563
pubmed: 28421057
Int J Med Microbiol. 2015 Dec;305(8):815-27
pubmed: 26372530
Clin Nutr ESPEN. 2017 Apr;18:37-43
pubmed: 29132736
J Dairy Sci. 2007 Oct;90(10):4513-23
pubmed: 17881672
Res Microbiol. 2017 Nov - Dec;168(9-10):782-792
pubmed: 28435139
MMWR Recomm Rep. 2015 Jun 5;64(RR-03):1-137
pubmed: 26042815
Front Microbiol. 2017 May 19;8:906
pubmed: 28579980
Res Microbiol. 2006 Oct;157(8):792-801
pubmed: 16844349
Front Microbiol. 2017 Feb 07;8:166
pubmed: 28223974
Nature. 2016 Jul 06;535(7610):85-93
pubmed: 27383983
J Microbiol Methods. 2016 Nov;130:123-128
pubmed: 27647064
Drugs. 2008;68(13):1787-802
pubmed: 18729533
BMC Microbiol. 2012 Oct 18;12:236
pubmed: 23078375
Trends Microbiol. 2018 Jan;26(1):16-32
pubmed: 28844447
Food Res Int. 2017 Oct;100(Pt 1):63-73
pubmed: 28873730
Am J Clin Nutr. 2014 Jun;99(6):1432-9
pubmed: 24646819
Appetite. 2008 Nov;51(3):456-67
pubmed: 18582508
Arch Gynecol Obstet. 2014 Mar;289(3):479-89
pubmed: 24170161
Anaerobe. 2017 Apr;44:3-12
pubmed: 28062270
BMC Womens Health. 2015 Dec 03;15:115
pubmed: 26635090
Front Microbiol. 2017 Jan 05;7:2116
pubmed: 28111569
PLoS One. 2015 Jun 22;10(6):e0131220
pubmed: 26098675
Crit Rev Food Sci Nutr. 2017 Oct 13;57(15):3233-3242
pubmed: 26505073
Arch Gynecol Obstet. 2015 Feb;291(2):251-7
pubmed: 25367602
J Dairy Sci. 2014 Sep;97(9):5898-903
pubmed: 25022690
AAPS PharmSciTech. 2014 Feb;15(1):29-43
pubmed: 24222267
Microb Ecol Health Dis. 2015 Aug 14;26:27799
pubmed: 26282697
Carbohydr Polym. 2016 Jan 20;136:1161-9
pubmed: 26572459
Eur J Obstet Gynecol Reprod Biol. 2012 Aug;163(2):210-5
pubmed: 22721635
PLoS Biol. 2015 Aug 18;13(8):e1002226
pubmed: 26284777
Gastroenterol Hepatol. 2017 Jun - Jul;40(6):417-429
pubmed: 28185664