Crystal structure of a Thermus aquaticus diversity-generating retroelement variable protein.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
09
2018
accepted:
21
12
2018
entrez:
11
1
2019
pubmed:
11
1
2019
medline:
29
9
2019
Statut:
epublish
Résumé
Diversity-generating retroelements (DGRs) are widely distributed in bacteria, archaea, and microbial viruses, and bring about unparalleled levels of sequence variation in target proteins. While DGR variable proteins share low sequence identity, the structures of several such proteins have revealed the C-type lectin (CLec)-fold as a conserved scaffold for accommodating massive sequence variation. This conservation has led to the suggestion that the CLec-fold may be useful in molecular surface display applications. Thermostability is an attractive feature in such applications, and thus we studied the variable protein of a DGR encoded by a prophage of the thermophile Thermus aquaticus. We report here the 2.8 Å resolution crystal structure of the variable protein from the T. aquaticus DGR, called TaqVP, and confirm that it has a CLec-fold. Remarkably, its variable region is nearly identical in structure to those of several other CLec-fold DGR variable proteins despite low sequence identity among these. TaqVP was found to be thermostable, which appears to be a property shared by several CLec-fold DGR variable proteins. These results provide impetus for the pursuit of the DGR variable protein CLec-fold in molecular display applications.
Identifiants
pubmed: 30629599
doi: 10.1371/journal.pone.0205618
pii: PONE-D-18-27522
pmc: PMC6328139
doi:
Substances chimiques
Bacterial Proteins
0
Recombinant Proteins
0
Retroelements
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0205618Subventions
Organisme : NIAID NIH HHS
ID : R01 AI096838
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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