A Meiotic Checkpoint Alters Repair Partner Bias to Permit Inter-sister Repair of Persistent DSBs.


Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
15 01 2019
Historique:
received: 11 05 2018
revised: 28 09 2018
accepted: 17 12 2018
entrez: 17 1 2019
pubmed: 17 1 2019
medline: 10 4 2020
Statut: ppublish

Résumé

Accurate meiotic chromosome segregation critically depends on the formation of inter-homolog crossovers initiated by double-strand breaks (DSBs). Inaccuracies in this process can drive aneuploidy and developmental defects, but how meiotic cells are protected from unscheduled DNA breaks remains unexplored. Here we define a checkpoint response to persistent meiotic DSBs in C. elegans that phosphorylates the synaptonemal complex (SC) to switch repair partner from the homolog to the sister chromatid. A key target of this response is the core SC component SYP-1, which is phosphorylated in response to ionizing radiation (IR) or unrepaired meiotic DSBs. Failure to phosphorylate (syp-1

Identifiants

pubmed: 30650366
pii: S2211-1247(18)32019-9
doi: 10.1016/j.celrep.2018.12.074
pmc: PMC6334227
pii:
doi:

Substances chimiques

DNA-Binding Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

775-787.e5

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Cancer Research UK
ID : FC0010048
Pays : United Kingdom
Organisme : Medical Research Council
ID : FC0010048
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC0010048
Pays : United Kingdom

Informations de copyright

Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

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Auteurs

Tatiana Garcia-Muse (T)

Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Av. Américo Vespucio 24, 41092 Seville, Spain; Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK. Electronic address: tatiana.muse@cabimer.es.

U Galindo-Diaz (U)

Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Av. Américo Vespucio 24, 41092 Seville, Spain.

M Garcia-Rubio (M)

Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Av. Américo Vespucio 24, 41092 Seville, Spain.

J S Martin (JS)

Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK.

J Polanowska (J)

Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK.

N O'Reilly (N)

DSB Repair Metabolism Laboratory, The Francis Crick Institute, Midland Road, London, UK.

A Aguilera (A)

Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Universidad de Sevilla-CSIC-Universidad Pablo de Olavide, Av. Américo Vespucio 24, 41092 Seville, Spain. Electronic address: aguilo@us.es.

Simon J Boulton (SJ)

Clare Hall Laboratories, Blanche Lane, South Mimms EN6 3LD, UK; DSB Repair Metabolism Laboratory, The Francis Crick Institute, Midland Road, London, UK. Electronic address: simon.boulton@crick.ac.uk.

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