Molecular identification, tissue distribution and functional analysis of somatostatin receptors (SSTRs) in red-spotted grouper (Epinephelus akaara).
Amino Acid Sequence
Animals
Bass
/ genetics
Female
Gene Expression Regulation
/ drug effects
HEK293 Cells
Humans
MAP Kinase Signaling System
/ drug effects
Phylogeny
Proto-Oncogene Proteins c-akt
/ metabolism
RNA, Messenger
/ genetics
Receptors, Somatostatin
/ chemistry
Reproducibility of Results
Sequence Analysis, Protein
Somatostatin
/ pharmacology
Tissue Distribution
/ drug effects
Red-spotted grouper
SSTR
Signaling pathways
Somatostatin
Journal
General and comparative endocrinology
ISSN: 1095-6840
Titre abrégé: Gen Comp Endocrinol
Pays: United States
ID NLM: 0370735
Informations de publication
Date de publication:
01 04 2019
01 04 2019
Historique:
received:
18
11
2018
revised:
05
01
2019
accepted:
10
01
2019
pubmed:
18
1
2019
medline:
19
3
2019
entrez:
18
1
2019
Statut:
ppublish
Résumé
In the present study, four full-length cDNAs of somatostatin receptor (sstr) were cloned from the forebrain and pituitary of red-spotted grouper. The four full-length cDNAs were designated 2292, 1522, 1873 and 1789 bp and identified as sstr1, sstr2, sstr3, and sstr5 by BLAST analysis; the corresponding sizes of the open reading frames (ORFs) were 1155, 1113, 1467 and 1503 bp, which encoding 384, 370, 488 and 500 aa, respectively. The four receptors have seven transmembrane structures and contain the YANSCANPI/VLY sequence, which is the conserved amino acid sequence of the SSTR family. A tissue distribution study showed that the four sstrs had different expression patterns, suggesting that they may play different roles in regulating different physiological processes. The four receptors mediate ERK1/2 phosphorylation by SS-14 in HEK293 cells, and SS-14 promotes ATK and ERK1/2 phosphorylation in primary hepatocytes of red-spotted grouper. These results facilitate the study of SSTRs-mediated intracellular signaling pathways.
Identifiants
pubmed: 30654020
pii: S0016-6480(18)30640-3
doi: 10.1016/j.ygcen.2019.01.007
pii:
doi:
Substances chimiques
RNA, Messenger
0
Receptors, Somatostatin
0
Somatostatin
51110-01-1
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
87-96Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.