cAMP Stimulates SLC26A3 Activity in Human Colon by a CFTR-Dependent Mechanism That Does Not Require CFTR Activity.

Caco-2 Cells Cell Differentiation / drug effects Chloride-Bicarbonate Antiporters / metabolism Colforsin / pharmacology Colon / metabolism Cyclic AMP / pharmacology Cystic Fibrosis Transmembrane Conductance Regulator / metabolism HEK293 Cells Humans Ion Transport Organoids / drug effects Reproducibility of Results Sulfate Transporters / metabolism
CFTR Cl(-)/HCO(3)(-) Exchange Colon Enteroids Secretory Diarrhea

Journal

Cellular and molecular gastroenterology and hepatology
ISSN: 2352-345X
Titre abrégé: Cell Mol Gastroenterol Hepatol
Pays: United States
ID NLM: 101648302

Informations de publication

Date de publication:
2019
Historique:
received: 20 08 2018
revised: 07 01 2019
accepted: 07 01 2019
pubmed: 20 1 2019
medline: 5 6 2019
entrez: 20 1 2019
Statut: ppublish

Résumé

SLC26A3 (DRA) is an electroneutral Cl This study re-evaluated cAMP regulation of DRA using new tools, including a successful knockout cell model, a specific DRA inhibitor (DRA DRA is an apical protein in human proximal colon, differentiated colonoid monolayers, and Caco-2 cells. It is glycosylated and appears as 2 bands. cAMP (forskolin) acutely stimulated DRA activity in human colonoids and Caco-2 cells. In these cells, DRA is the predominant apical Cl DRA is acutely stimulated by cAMP by a process that is CFTR-dependent, but appears to be one of multiple regulatory effects of CFTR that does not require CFTR activity.

Sections du résumé

BACKGROUND & AIMS
SLC26A3 (DRA) is an electroneutral Cl
METHODS
This study re-evaluated cAMP regulation of DRA using new tools, including a successful knockout cell model, a specific DRA inhibitor (DRA
RESULTS
DRA is an apical protein in human proximal colon, differentiated colonoid monolayers, and Caco-2 cells. It is glycosylated and appears as 2 bands. cAMP (forskolin) acutely stimulated DRA activity in human colonoids and Caco-2 cells. In these cells, DRA is the predominant apical Cl
CONCLUSIONS
DRA is acutely stimulated by cAMP by a process that is CFTR-dependent, but appears to be one of multiple regulatory effects of CFTR that does not require CFTR activity.

Identifiants

DOI: 10.1016/j.jcmgh.2019.01.002 PMID: 30659943 PMC: PMC6438990
pubmed: 30659943
pii: S2352-345X(19)30003-7
doi: 10.1016/j.jcmgh.2019.01.002
pmc: PMC6438990
pii:
doi:

Substances chimiques

Chloride-Bicarbonate Antiporters 0
SLC26A3 protein, human 0
SLC26A6 protein, human 0
Sulfate Transporters 0
Cystic Fibrosis Transmembrane Conductance Regulator 126880-72-6
Colforsin 1F7A44V6OU
Cyclic AMP E0399OZS9N

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

641-653

Subventions

Organisme : NIDDK NIH HHS
ID : R24 DK099803
Pays : United States
Organisme : NIDDK NIH HHS
ID : P01 DK072084
Pays : United States
Organisme : NIDDK NIH HHS
ID : R24 DK064388
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK061931
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR000003
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK068271
Pays : United States
Organisme : NCATS NIH HHS
ID : U18 TR000552
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK116352
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK089502
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK061765
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK034854
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK026523
Pays : United States

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

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Auteurs

Chung-Ming Tse (CM)

Department of Medicine, Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Jianyi Yin (J)

Department of Medicine, Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Varsha Singh (V)

Department of Medicine, Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Rafiquel Sarker (R)

Department of Medicine, Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Ruxian Lin (R)

Department of Medicine, Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland.

Alan S Verkman (AS)

Department of Medicine, Department of Physiology, University of California San Francisco, San Francisco, California.

Jerrold R Turner (JR)

Department of Pathology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.

Mark Donowitz (M)

Department of Medicine, Division of Gastroenterology and Hepatology, Johns Hopkins University School of Medicine, Baltimore, Maryland; Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland. Electronic address: mdonowit@jhmi.edu.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules Caco-2 › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Protéines transporteurs de solutés › Protéines SLC4A › Antiporteurs des ions chlorure-bicarbonate › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Terpènes › Diterpènes › Colforsine › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Système digestif › Tube digestif › Tube digestif inférieur › Gros intestin › Côlon › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › AMP cyclique › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Pompes ioniques › Transporteurs d'anions › Transporteurs d'anions organiques › Transporteurs d'anions organiques ATP-dépendants › Protéines associées à la multirésistance aux médicaments › Protéine CFTR › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Métabolisme › Transport biologique › Transport des ions › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Tissus › Organoïdes › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › cAMP Stimulates SLC26A3 Activity in Human...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Protéines transporteurs de solutés › Transporteurs de sulfate › cAMP Stimulates SLC26A3 Activity in Human...