Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation.
Acetylation
Alternative Splicing
/ radiation effects
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Darkness
Gene Expression Regulation, Plant
/ radiation effects
Histones
/ genetics
Kinetics
Light
Mutation
Plants, Genetically Modified
/ genetics
RNA Polymerase II
/ genetics
RNA, Plant
/ biosynthesis
Transcription Elongation, Genetic
/ radiation effects
Transcriptional Elongation Factors
/ genetics
alternative splicing
light control in plants
transcription elongation
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
07 03 2019
07 03 2019
Historique:
received:
18
06
2018
revised:
19
10
2018
accepted:
07
12
2018
pubmed:
22
1
2019
medline:
25
6
2019
entrez:
22
1
2019
Statut:
ppublish
Résumé
Light makes carbon fixation possible, allowing plant and animal life on Earth. We have previously shown that light regulates alternative splicing in plants. Light initiates a chloroplast retrograde signaling that regulates nuclear alternative splicing of a subset of Arabidopsis thaliana transcripts. Here, we show that light promotes RNA polymerase II (Pol II) elongation in the affected genes, whereas in darkness, elongation is lower. These changes in transcription are consistent with elongation causing the observed changes in alternative splicing, as revealed by different drug treatments and genetic evidence. The light control of splicing and elongation is abolished in an Arabidopsis mutant defective in the transcription factor IIS (TFIIS). We report that the chloroplast control of nuclear alternative splicing in plants responds to the kinetic coupling mechanism found in mammalian cells, providing unique evidence that coupling is important for a whole organism to respond to environmental cues.
Identifiants
pubmed: 30661982
pii: S1097-2765(18)31035-9
doi: 10.1016/j.molcel.2018.12.005
pii:
doi:
Substances chimiques
Arabidopsis Proteins
0
Histones
0
RNA, Plant
0
Transcriptional Elongation Factors
0
transcription factor S-II
0
RNA Polymerase II
EC 2.7.7.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1066-1074.e3Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.