Whole blood RNA sequencing reveals a unique transcriptomic profile in patients with ARDS following hematopoietic stem cell transplantation.
Acute respiratory distress syndrome (ARDS)
Bone marrow transplant
Hematopoietic stem cell transplantation
RNA sequencing
RNA-Seq
Respiratory failure
Transcriptome profiling
Journal
Respiratory research
ISSN: 1465-993X
Titre abrégé: Respir Res
Pays: England
ID NLM: 101090633
Informations de publication
Date de publication:
21 Jan 2019
21 Jan 2019
Historique:
received:
27
07
2018
accepted:
08
01
2019
entrez:
23
1
2019
pubmed:
23
1
2019
medline:
19
3
2019
Statut:
epublish
Résumé
The acute respiratory distress syndrome (ARDS) is characterized by the acute onset of hypoxemia and bilateral lung infiltrates in response to an inciting event, and is associated with high morbidity and mortality. Patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) are at increased risk for ARDS. We hypothesized that HSCT patients with ARDS would have a unique transcriptomic profile identifiable in peripheral blood compared to those that did not undergo HSCT. We isolated RNA from banked peripheral blood samples from a biorepository of critically ill ICU patients. RNA-Seq was performed on 11 patients with ARDS (5 that had undergone HSCT and 6 that had not) and 12 patients with sepsis without ARDS (5 that that had undergone HCST and 7 that had not). We identified 687 differentially expressed genes between ARDS and ARDS-HSCT (adjusted p-value < 0.01), including IFI44L, OAS3, LY6E, and SPATS2L that had increased expression in ARDS vs. ARDS-HSCT; these genes were not differentially expressed in sepsis vs sepsis-HSCT. Gene ontology enrichment analysis revealed that many differentially expressed genes were related to response to type I interferon. Our findings reveal significant differences in whole blood transcriptomic profiles of patients with non-HSCT ARDS compared to ARDS-HSCT patients and point toward different immune responses underlying ARDS and ARDS-HSCT that contribute to lung injury.
Sections du résumé
BACKGROUND
BACKGROUND
The acute respiratory distress syndrome (ARDS) is characterized by the acute onset of hypoxemia and bilateral lung infiltrates in response to an inciting event, and is associated with high morbidity and mortality. Patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) are at increased risk for ARDS. We hypothesized that HSCT patients with ARDS would have a unique transcriptomic profile identifiable in peripheral blood compared to those that did not undergo HSCT.
METHODS
METHODS
We isolated RNA from banked peripheral blood samples from a biorepository of critically ill ICU patients. RNA-Seq was performed on 11 patients with ARDS (5 that had undergone HSCT and 6 that had not) and 12 patients with sepsis without ARDS (5 that that had undergone HCST and 7 that had not).
RESULTS
RESULTS
We identified 687 differentially expressed genes between ARDS and ARDS-HSCT (adjusted p-value < 0.01), including IFI44L, OAS3, LY6E, and SPATS2L that had increased expression in ARDS vs. ARDS-HSCT; these genes were not differentially expressed in sepsis vs sepsis-HSCT. Gene ontology enrichment analysis revealed that many differentially expressed genes were related to response to type I interferon.
CONCLUSIONS
CONCLUSIONS
Our findings reveal significant differences in whole blood transcriptomic profiles of patients with non-HSCT ARDS compared to ARDS-HSCT patients and point toward different immune responses underlying ARDS and ARDS-HSCT that contribute to lung injury.
Identifiants
pubmed: 30665420
doi: 10.1186/s12931-019-0981-6
pii: 10.1186/s12931-019-0981-6
pmc: PMC6341764
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
15Subventions
Organisme : NIGMS NIH HHS
ID : K08 GM102695
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL141992
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL007586
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL135142
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL133433
Pays : United States
Organisme : NHLBI NIH HHS
ID : R56 HL142767
Pays : United States
Organisme : NHLBI NIH HHS
ID : K08 HL143197
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL112747
Pays : United States
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