FACT and Ubp10 collaborate to modulate H2B deubiquitination and nucleosome dynamics.
Alleles
DNA Replication
/ drug effects
DNA-Binding Proteins
/ metabolism
Gene Expression Regulation, Fungal
/ drug effects
High Mobility Group Proteins
/ metabolism
Histones
/ metabolism
Hydroxyurea
/ pharmacology
Mutation
/ genetics
Nucleosomes
/ drug effects
Phenotype
Promoter Regions, Genetic
/ genetics
Protein Binding
/ drug effects
Saccharomyces cerevisiae
/ drug effects
Saccharomyces cerevisiae Proteins
/ metabolism
Transcription, Genetic
/ drug effects
Transcriptional Elongation Factors
/ metabolism
Ubiquitin
/ metabolism
Ubiquitin Thiolesterase
/ metabolism
Ubiquitination
/ drug effects
DNA replication
S. cerevisiae
biochemistry
chemical biology
chromosomes
deubiquitinating enzyme
gene expression
histone chaperone
nucleosome dynamics
transcription
ubiquitin
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
25 01 2019
25 01 2019
Historique:
received:
10
08
2018
accepted:
24
01
2019
pubmed:
27
1
2019
medline:
11
4
2020
entrez:
26
1
2019
Statut:
epublish
Résumé
Monoubiquitination of histone H2B (H2B-Ub) plays a role in transcription and DNA replication, and is required for normal localization of the histone chaperone, FACT. In yeast, H2B-Ub is deubiquitinated by Ubp8, a subunit of SAGA, and Ubp10. Although they target the same substrate, loss of Ubp8 and Ubp10 cause different phenotypes and alter the transcription of different genes. We show that Ubp10 has poor activity on yeast nucleosomes, but that the addition of FACT stimulates Ubp10 activity on nucleosomes and not on other substrates. Consistent with a role for FACT in deubiquitinating H2B in vivo, a FACT mutant strain shows elevated levels of H2B-Ub. Combination of FACT mutants with deletion of Ubp10, but not Ubp8, confers increased sensitivity to hydroxyurea and activates a cryptic transcription reporter, suggesting that FACT and Ubp10 may coordinate nucleosome assembly during DNA replication and transcription. Our findings reveal unexpected interplay between H2B deubiquitination and nucleosome dynamics.
Identifiants
pubmed: 30681413
doi: 10.7554/eLife.40988
pii: 40988
pmc: PMC6372288
doi:
pii:
Substances chimiques
DNA-Binding Proteins
0
FACT protein, S cerevisiae
0
High Mobility Group Proteins
0
Histones
0
Nucleosomes
0
Saccharomyces cerevisiae Proteins
0
Transcriptional Elongation Factors
0
Ubiquitin
0
UBP10 protein, S cerevisiae
EC 3.4.19.12
Ubiquitin Thiolesterase
EC 3.4.19.12
Hydroxyurea
X6Q56QN5QC
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM064649
Pays : United States
Organisme : NIGMS NIH HHS
ID : GM095822
Pays : United States
Organisme : NIGMS NIH HHS
ID : GM064649
Pays : United States
Organisme : NIGMS NIH HHS
ID : Training Grant GM008403
Pays : United States
Organisme : Israel Council of Higher Education
ID : Fellowship
Pays : International
Organisme : National Science Foundation
ID : Graduate Research Fellowship
Pays : International
Organisme : NIGMS NIH HHS
ID : R01 GM095822
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008403
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130393
Pays : United States
Informations de copyright
© 2019, Nune et al.
Déclaration de conflit d'intérêts
MN, MM, ZC, LM, MJ, HS, AB No competing interests declared, TF, CW Reviewing editor, eLife
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