Impact of frit dispersion on gradient performance in high-throughput liquid chromatography.
Frit dispersion
Gradient peak capacity
High-throughput gradient liquid chromatography
Post-column dispersion
Short narrow-bore columns
Sub-2 μm superficially porous particles
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
26 Apr 2019
26 Apr 2019
Historique:
received:
27
11
2018
revised:
05
01
2019
accepted:
08
01
2019
pubmed:
29
1
2019
medline:
11
5
2019
entrez:
29
1
2019
Statut:
ppublish
Résumé
The impact of porous frits (2.1 mm i.d. × 1 mm thick, 0.2 μm porosity, 20% void, 0.69 μL) in short 0.5-5 cm long × 2.1 mm i.d. columns packed with sub-2 μm superficially porous particles on gradient performance was investigated using a low dispersive i-class ACQUITY UPLC system (25 cm × 75 μm outlet tube + 250 nL optical cell). In order to maximize data accuracy, the sample dispersion through a single frit was measured from four independent methods: (1) plate height subtraction, (2) peak capacity versus efficiency plot, (3) flow reversal, and (4) direct measurement. Frit dispersion increases non-linearly with increasing flow rate. The corresponding volume variances of small molecules were measured at 0.16 ± 0.04, 0.24 ± 0.05, and 0.31 ± 0.06 μL
Identifiants
pubmed: 30686649
pii: S0021-9673(19)30034-2
doi: 10.1016/j.chroma.2019.01.021
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110-119Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.