Lessons from human umbilical cord: gender differences in stem cells from Wharton's jelly.
Cell Differentiation
DNA (Cytosine-5-)-Methyltransferase 1
/ metabolism
Female
Gene Expression
/ physiology
Genes, myc
/ physiology
Healthy Volunteers
Humans
Male
Mesenchymal Stem Cells
/ metabolism
Nanog Homeobox Protein
/ metabolism
Octamer Transcription Factor-3
/ metabolism
Pregnancy
SOXB1 Transcription Factors
/ metabolism
Sex Characteristics
Umbilical Cord
/ cytology
Vagina
Wharton Jelly
/ cytology
Gender
Stem cells
Wharton jelly
epigenetics
pluripotency
Journal
European journal of obstetrics, gynecology, and reproductive biology
ISSN: 1872-7654
Titre abrégé: Eur J Obstet Gynecol Reprod Biol
Pays: Ireland
ID NLM: 0375672
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
29
09
2018
revised:
12
12
2018
accepted:
17
12
2018
pubmed:
29
1
2019
medline:
6
6
2019
entrez:
29
1
2019
Statut:
ppublish
Résumé
To study the molecular features of mesenchymal stem cells from Wharton Jelly (WJ-MSCs) of umbilical cord to predict their differentiation capacity. Comparison of gene expression from mesenchymal stem cells of male and female umbilical cord SETTING: University hospital PATIENT (S): umbilical cords (n = 12, 6 males and 6 females) retrieved from spontaneous full-term vaginal delivery of healthy women INTERVENTION: we analyzed the expression of the stemness related genes C-MYC, OCT4, SOX2 and NANOG and of the epigenetic modulating gene DNA-methyltransferase 1 (DNMT1). WJ-MSCs were isolated by standard procedures and immunophenotypically characterized. Gene expression analysis of stemness related genes and the epigenetic modulating gene DNMT1 were performed by real-time PCR RESULTS: expression of the OCT4 and DNMT1 genes was significantly higher in WJ- MSCs isolated from male subjects, as compared to MSCs isolated from female-derived WJ. The resulting higher expression of OCT4 and DNMT1 in WJ-MSCs from males as compared with female WJ-MSCs for the first time identifies a specific relationship between stemness genes, an epigenetic modulator, and gender differences. our findings disclose novel biomedical implications in WJ-MSCs related to the sex of the donor, thus providing additional cues to exploit their regenerative potential in allogenic transplantation.
Identifiants
pubmed: 30690190
pii: S0301-2115(19)30004-1
doi: 10.1016/j.ejogrb.2018.12.028
pii:
doi:
Substances chimiques
NANOG protein, human
0
Nanog Homeobox Protein
0
Octamer Transcription Factor-3
0
POU5F1 protein, human
0
SOX2 protein, human
0
SOXB1 Transcription Factors
0
DNA (Cytosine-5-)-Methyltransferase 1
EC 2.1.1.37
DNMT1 protein, human
EC 2.1.1.37
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
143-148Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.