Electrochemical characterization of LHCII on graphite electrodes - Potential-dependent photoactivation and arrangement of complexes.
Adsorption
Electrochemical Techniques
Electrodes
Electron Transport
Graphite
/ chemistry
Immobilized Proteins
/ chemistry
Light
Light-Harvesting Protein Complexes
/ chemistry
Oxidation-Reduction
Photosystem II Protein Complex
/ chemistry
Plant Leaves
/ chemistry
Plant Proteins
/ chemistry
Spinacia oleracea
/ chemistry
77 K fluorescence
Chlorophyll-protein complexes
Electrochemistry
Graphite electrode
LHCII
Photocurrent
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
18
08
2018
revised:
14
01
2019
accepted:
14
01
2019
pubmed:
29
1
2019
medline:
18
4
2019
entrez:
29
1
2019
Statut:
ppublish
Résumé
Light-dependent electrochemical properties of the light harvesting complexes of Photosystem II (LHCII) and the corresponding interactions with screen-printed graphite electrodes (GEs) are determined. No exogenous soluble redox mediators are used. LHCII isolated from spinach leaves are immobilized on GE by physical adsorption and through interactions with glutaraldehyde. Importantly, the insertion of LHCII into the pores of a GE is achieved by subjecting the electrode to specific potentials. Both trimeric and aggregated forms of LHCII located within the graphite layer retain their native structures. Voltammetric current peaks centred at ca. -230 and + 50 mV vs. Ag/AgCl (+94 and + 374 mV vs. NHE) limit the investigation of the reduction and oxidation processes of immobilized LHCII. An anodic photocurrent is generated in the LHCII-GE proportional to light intensity and can reach a value of 150 nA/cm
Identifiants
pubmed: 30690422
pii: S1567-5394(18)30372-4
doi: 10.1016/j.bioelechem.2019.01.005
pii:
doi:
Substances chimiques
Immobilized Proteins
0
Light-Harvesting Protein Complexes
0
Photosystem II Protein Complex
0
Plant Proteins
0
Graphite
7782-42-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
37-48Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.