A next generation sequencing approach for targeted Varroa destructor (Acari: Varroidae) mitochondrial DNA analysis based on honey derived environmental DNA.
Honey bee
Lineages
NGS
Parasite
Variants
eDNA
Journal
Journal of invertebrate pathology
ISSN: 1096-0805
Titre abrégé: J Invertebr Pathol
Pays: United States
ID NLM: 0014067
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
05
06
2018
revised:
26
01
2019
accepted:
28
01
2019
pubmed:
2
2
2019
medline:
15
5
2020
entrez:
2
2
2019
Statut:
ppublish
Résumé
Honey contains DNA from many different organisms that are part of hive micro-environmental niches and honey bee pathospheres. In this study, we recovered and sequenced mite mitochondrial DNA (mtDNA) from honey from different locations around the world (Europe, Asia, Africa, North and South America). DNA extracted from 17 honey samples was amplified with eight primer pairs targeting three mite mtDNA genes, obtaining 88 amplicons that were sequenced with an Ion Torrent sequencing platform. A bioinformatic pipeline compared produced reads with Varroa spp. mtDNA sequence entries available in GenBank and assigned them to different mitotypes. In all honey samples, the highest percentage of reads was attributed to the K1 lineage, including a few variants derived from it, in addition to J1 reads observed in the two South American samples and C1-1 reads obtained from the Chinese honey. This study opens new possibilities to analyse mite lineages and variants and monitor their geographical and temporal distribution, simplifying surveillance against this damaging honey bee parasite.
Identifiants
pubmed: 30707918
pii: S0022-2011(18)30194-0
doi: 10.1016/j.jip.2019.01.005
pii:
doi:
Substances chimiques
DNA, Environmental
0
DNA, Mitochondrial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
47-53Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.