[Detection of ALK and ROS1 rearrangements by immunocytochemistry on cytological samples].
Mise en évidence du réarrangement d’ALK et ROS1 en immunocytochimie sur liquides de ponction.
Adenocarcinoma
/ genetics
Adult
Aged
Aged, 80 and over
Anaplastic Lymphoma Kinase
/ genetics
Female
Gene Rearrangement
Genes, Neoplasm
/ genetics
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Liquid Biopsy
Lung Neoplasms
/ genetics
Male
Middle Aged
Protein-Tyrosine Kinases
/ genetics
Proto-Oncogene Proteins
/ genetics
ALK
Adénocarcinome bronchique
Cancer du poumon
Cytologie
Cytology
Immunocytochemistry
Immunocytochimie
Lung adenocarcinoma
Lung cancer
ROS1
Journal
Annales de pathologie
ISSN: 0242-6498
Titre abrégé: Ann Pathol
Pays: France
ID NLM: 8106337
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
received:
20
07
2018
revised:
31
10
2018
accepted:
09
12
2018
pubmed:
3
2
2019
medline:
7
1
2020
entrez:
3
2
2019
Statut:
ppublish
Résumé
The identification of ALK and ROS1 rearrangements has become essential for the theranostic management of patients with non-small cell lung cancer, especially in stage IV or inoperable patients. These testings are now performed by immunohistochemistry on histological samples and confirmed by fluorescent in situ hybridization in case of positive or doubtful results. The diagnosis of lung cancer is often performed at an advanced or metastatic stage and cytological sample could be the only material containing malignant cells available at these stages. Therefore, the detection of ALK and ROS1 rearrangement by immunocytochemical analysis on cytological specimens is needed. We performed this test on 27 cytological samples of lung adenocarcinomas, and we compared our results with several other techniques: on the same sample or on biopsy in another laboratory, on the same sample by fluorescent in situ hybridization and/or immunochemistry. We found a very good concordance between all these techniques, thus validating our immunocytochemical method on cytological samples according to the ISO 15189 norm.
Identifiants
pubmed: 30709602
pii: S0242-6498(18)30187-1
doi: 10.1016/j.annpat.2018.12.003
pii:
doi:
Substances chimiques
Proto-Oncogene Proteins
0
ALK protein, human
EC 2.7.10.1
Anaplastic Lymphoma Kinase
EC 2.7.10.1
Protein-Tyrosine Kinases
EC 2.7.10.1
ROS1 protein, human
EC 2.7.10.1
Types de publication
Journal Article
Langues
fre
Sous-ensembles de citation
IM
Pagination
227-236Informations de copyright
Copyright © 2018 Elsevier Masson SAS. All rights reserved.