Structural and biomechanical changes to dentin extracellular matrix following chemical removal of proteoglycans.

Collagen Type I Dentin Extracellular Matrix Microscopy, Electron, Transmission Proteoglycans
Collagen Dentin Histology Modulus of elasticity Proteoglycans

Journal

Odontology
ISSN: 1618-1255
Titre abrégé: Odontology
Pays: Japan
ID NLM: 101134822

Informations de publication

Date de publication:
Jul 2019
Historique:
received: 08 05 2018
accepted: 24 11 2018
pubmed: 3 2 2019
medline: 27 11 2019
entrez: 3 2 2019
Statut: ppublish

Résumé

Proteoglycans are biomacromolecules with significant biomineralization and structural roles in the dentin extracellular matrix. This study comprehensively assessed the mechanical properties and morphology of the dentin extracellular matrix following chemical removal of proteoglycans to elucidate the structural roles of proteoglycans in dentin. Dentin extracellular matrix was prepared from extracted teeth after complete tissue demineralization. Chemical removal of proteoglycans was carried-out using guanidine hydrochloride for up to 10 days. The removal of proteoglycans was determined by dimethylmethylene blue colorimetric assay and histological staining analyses using transmission electron microscopy and optical microscopy. The modulus of elasticity of dentin matrix was determined by a 3-point bending test method. Partial removal of proteoglycans induced significant modifications to the dentin matrix, particularly to type I collagen. Removal of proteoglycans significantly decreased the modulus of elasticity of dentin extracellular matrix (p < 0.0001). In conclusion, the subtle disruption of proteoglycans induces pronounced changes to the collagen network packing and the bulk modulus of elasticity of dentin matrix.

Identifiants

DOI: 10.1007/s10266-018-00408-0 PMID: 30710179 PMC: PMC6559829
pubmed: 30710179
doi: 10.1007/s10266-018-00408-0
pii: 10.1007/s10266-018-00408-0
pmc: PMC6559829
mid: NIHMS1525001
doi:

Substances chimiques

Collagen Type I 0
Proteoglycans 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

316-323

Subventions

Organisme : NIDCR NIH HHS
ID : R01 DE021040
Pays : United States
Organisme : National Institute of Dental and Craniofacial Research
ID : DE21040
Organisme : Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (BR)
ID : BEX 17764/12-2

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Auteurs

Ana Paula Farina (AP)

Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Room 531, Chicago, IL, 60612, USA.
Department of Restorative Dentistry, Passo Fundo Dental School, University of Passo Fundo, UPF, Passo Fundo, RS, Brazil.

Cristina M P Vidal (CMP)

Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Room 531, Chicago, IL, 60612, USA.
Department of Operative Dentistry, College of Dentistry, University of Iowa, Iowa City, IA, USA.

Doglas Cecchin (D)

Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Room 531, Chicago, IL, 60612, USA.
Department of Restorative Dentistry, Passo Fundo Dental School, University of Passo Fundo, UPF, Passo Fundo, RS, Brazil.

Thaiane R Aguiar (TR)

Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Room 531, Chicago, IL, 60612, USA.
Department of Clinical Dentistry, School of Dentistry, Federal University of Bahia, Salvador, BA, Brazil.

Ana K Bedran-Russo (AK)

Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina Street, Room 531, Chicago, IL, 60612, USA. bedran@uic.edu.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Collagène › Collagènes fibrillaires › Collagène de type I › Structural and biomechanical changes to dentin...
questionsmedicales.fr › Système stomatognathique › Bouche › Denture › Composants dentaires › Dentine › Structural and biomechanical changes to dentin...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Matrice extracellulaire › Structural and biomechanical changes to dentin...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à transmission › Structural and biomechanical changes to dentin...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Glycoprotéines › Protéoglycanes › Structural and biomechanical changes to dentin...