Single-cell RNA sequencing reveals midbrain dopamine neuron diversity emerging during mouse brain development.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
04 02 2019
04 02 2019
Historique:
received:
28
06
2018
accepted:
10
01
2019
entrez:
6
2
2019
pubmed:
6
2
2019
medline:
11
4
2019
Statut:
epublish
Résumé
Midbrain dopamine (mDA) neurons constitute a heterogenous group of cells that have been intensely studied, not least because their degeneration causes major symptoms in Parkinson's disease. Understanding the diversity of mDA neurons - previously well characterized anatomically - requires a systematic molecular classification at the genome-wide gene expression level. Here, we use single cell RNA sequencing of isolated mouse neurons expressing the transcription factor Pitx3, a marker for mDA neurons. Analyses include cells isolated during development up until adulthood and the results are validated by histological characterization of newly identified markers. This identifies seven neuron subgroups divided in two major branches of developing Pitx3-expressing neurons. Five of them express dopaminergic markers, while two express glutamatergic and GABAergic markers, respectively. Analysis also indicate evolutionary conservation of diversity in humans. This comprehensive molecular characterization will provide a valuable resource for elucidating mDA neuron subgroup development and function in the mammalian brain.
Identifiants
pubmed: 30718509
doi: 10.1038/s41467-019-08453-1
pii: 10.1038/s41467-019-08453-1
pmc: PMC6362095
doi:
Substances chimiques
Homeodomain Proteins
0
Transcription Factors
0
homeobox protein PITX3
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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