A Membrane-Bound Gluconate Dehydrogenase from 2-Keto-D-Gluconic Acid Industrial Producing Strain Pseudomonas plecoglossicida JUIM01: Purification, Characterization, and Gene Identification.
2-Keto-D-gluconic acid (2KGA)
Gluconate dehydrogenase
Heterologous expression
Pseudomonas plecoglossicida
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
05
11
2018
accepted:
11
01
2019
pubmed:
8
2
2019
medline:
8
8
2019
entrez:
8
2
2019
Statut:
ppublish
Résumé
The membrane-bound gluconate dehydrogenase (mGADH) is a critical enzyme for 2-keto-D-gluconic acid (2KGA) production in Pseudomonas plecoglossicida JUIM01. The purified native flavin adenine dinucleotide-dependent mGADH (FAD-mGADH) was consisted of a gamma subunit, a flavoprotein subunit, and a cytochrome c subunit with molecular mass of ~ 27, 65, and 47 kDa, respectively. The specific activity of FAD-mGADH was determined as 90.71 U/mg at optimum pH and temperature of 6.0 and 35 °C. The K
Identifiants
pubmed: 30729393
doi: 10.1007/s12010-019-02951-0
pii: 10.1007/s12010-019-02951-0
doi:
Substances chimiques
Flavoproteins
0
Gluconates
0
Flavin-Adenine Dinucleotide
146-14-5
Cytochromes c
9007-43-6
Oxidoreductases
EC 1.-
gluconic acid
R4R8J0Q44B
Types de publication
Journal Article
Langues
eng
Pagination
897-913Subventions
Organisme : National Natural Science Foundation of China
ID : 31571885
Organisme : the Innovation Group Construction Program of Jiangxi Province
ID : 20142BCB24024
Organisme : Science & Technology Program of Jiangxi Province
ID : [2015]64
Organisme : Science & Technology Program of Dexing city
ID : [2015]44
Organisme : the National First-class Discipline Program of Light Industry Technology and Engineering
ID : LITE2018-11
Organisme : the National First-class Discipline Program of Light Industry Technology and Engineering
ID : LITE2018-18