Molecular identification of Wolbachia and Sodalis glossinidius in the midgut of Glossina fuscipes quanzensis from the Democratic Republic of Congo.
Identification moléculaire de Wolbachia sp et Sodalis glossinidius dans l’intestin moyen de Glossina fuscipes quanzensis de la République Démocratique du Congo.
Animals
Coinfection
/ microbiology
DNA, Bacterial
/ genetics
Democratic Republic of the Congo
Digestive System
/ microbiology
Enterobacteriaceae
/ genetics
Fructose-Bisphosphate Aldolase
/ genetics
High-Throughput Nucleotide Sequencing
Insect Vectors
/ microbiology
Polymerase Chain Reaction
Symbiosis
Tsetse Flies
/ microbiology
Wolbachia
/ genetics
Journal
Parasite (Paris, France)
ISSN: 1776-1042
Titre abrégé: Parasite
Pays: France
ID NLM: 9437094
Informations de publication
Date de publication:
2019
2019
Historique:
received:
13
09
2018
accepted:
23
01
2019
entrez:
8
2
2019
pubmed:
8
2
2019
medline:
20
2
2019
Statut:
ppublish
Résumé
During the last 30 years, investigations on the microbiome of different tsetse species have generated substantial data on the bacterial flora of these cyclical vectors of African trypanosomes, with the overarching goal of improving the control of trypanosomiases. It is in this context that the presence of Wolbachia and Sodalis glossinidius was studied in wild populations of Glossina fuscipes quanzensis from the Democratic Republic of Congo. Tsetse flies were captured with pyramidal traps. Of the 700 Glossina f. quanzensis captured, 360 were dissected and their midguts collected and analyzed. Sodalis glossinidius and Wolbachia were identified by PCR. The Wolbachia-positive samples were genetically characterized with five molecular markers. PCR revealed 84.78% and 15.55% midguts infected by Wolbachia and S. glossinidius, respectively. The infection rates varied according to capture sites. Of the five molecular markers used to characterize Wolbachia, only the fructose bis-phosphate aldolase gene was amplified for about 60% of midguts previously found with Wolbachia infections. The sequencing results confirmed the presence of Wolbachia and revealed the presence of S. glossinidius in the midgut of Glossina f. quanzensis. A low level of midguts were naturally co-infected by both bacteria. The data generated in this study open a framework for investigations aimed at understanding the contribution of these symbiotic microorganisms to the vectorial competence of Glossina fuscipes quanzensis.
Identifiants
pubmed: 30729921
doi: 10.1051/parasite/2019005
pii: parasite180128
pmc: PMC6366345
doi:
Substances chimiques
DNA, Bacterial
0
Fructose-Bisphosphate Aldolase
EC 4.1.2.13
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5Informations de copyright
© G. Simo et al., published by EDP Sciences, 2019.
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