In vivo RNA editing of point mutations via RNA-guided adenosine deaminases.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
16
08
2018
accepted:
13
12
2018
pubmed:
10
2
2019
medline:
23
5
2019
entrez:
10
2
2019
Statut:
ppublish
Résumé
We present in vivo sequence-specific RNA base editing via adenosine deaminases acting on RNA (ADAR) enzymes with associated ADAR guide RNAs (adRNAs). To achieve this, we systematically engineered adRNAs to harness ADARs, and comprehensively evaluated the specificity and activity of the toolsets in vitro and in vivo via two mouse models of human disease. We anticipate that this platform will enable tunable and reversible engineering of cellular RNAs for diverse applications.
Identifiants
pubmed: 30737497
doi: 10.1038/s41592-019-0323-0
pii: 10.1038/s41592-019-0323-0
pmc: PMC6395520
mid: NIHMS1516811
doi:
Substances chimiques
RNA, Guide
0
RNA, Messenger
0
Adenosine Deaminase
EC 3.5.4.4
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
239-242Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM123313
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA222826
Pays : United States
Organisme : NIDDK NIH HHS
ID : K01 DK119687
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG009285
Pays : United States
Organisme : NIDDK NIH HHS
ID : F32 DK112682
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : CommentIn
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