A novel endo-β-1,4-xylanase from Pediococcus acidilactici GC25; purification, characterization and application in clarification of fruit juices.
Pediococsus acidilactici
Purification
Xylanase
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 May 2019
15 May 2019
Historique:
received:
14
11
2018
revised:
11
01
2019
accepted:
08
02
2019
pubmed:
13
2
2019
medline:
13
7
2019
entrez:
13
2
2019
Statut:
ppublish
Résumé
A novel extracellular xylanase was purified and characterized from Pediococcus acidilactici GC25 (GenBank number: MF289522). The purification was 4.6-fold with a yield of 43.61% through acetone precipitation, Q-Sepharose, and CM-Sepharose ion change chromatography. The molecular weight of the enzyme was 48.15 kDa, and the optimum pH and temperature were 7.0 and 40 °C, respectively. The maximum activity was observed between 20 and 50 °C. Although it was active within a wide pH range (pH 2.0-9.0), it retained over 85% of its activity after 24 h incubation; and over 70% of its activity after 168 h incubation in neutral and alkaline pH. It was observed that the enzyme showed high stability with K
Identifiants
pubmed: 30753879
pii: S0141-8130(18)36225-1
doi: 10.1016/j.ijbiomac.2019.02.054
pii:
doi:
Substances chimiques
Metals
0
Endo-1,4-beta Xylanases
EC 3.2.1.8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
571-578Informations de copyright
Copyright © 2019. Published by Elsevier B.V.