Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.
Actins
/ metabolism
Animals
Animals, Genetically Modified
Body Patterning
Cadherins
/ metabolism
Cell Membrane
/ metabolism
Cell Movement
Epithelium
/ embryology
Gene Expression Regulation
Gene Expression Regulation, Developmental
Green Fluorescent Proteins
/ metabolism
Heterocyclic Compounds, 4 or More Rings
/ pharmacology
Microscopy, Confocal
Morphogenesis
Myosin Type II
/ metabolism
Myosins
/ metabolism
Neural Crest
/ embryology
Neural Plate
/ embryology
Oligonucleotides
/ pharmacology
Prospective Studies
Zebrafish
/ embryology
Zebrafish Proteins
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
12 02 2019
12 02 2019
Historique:
received:
04
06
2018
accepted:
07
12
2018
entrez:
14
2
2019
pubmed:
14
2
2019
medline:
24
9
2020
Statut:
epublish
Résumé
Tissue internalisation is a key morphogenetic mechanism by which embryonic tissues generate complex internal organs and a number of studies of epithelia have outlined a general view of tissue internalisation. Here we have used quantitative live imaging and mutant analysis to determine whether similar mechanisms are responsible for internalisation in a tissue that apparently does not have a typical epithelial organisation - the zebrafish neural plate. We found that although zebrafish embryos begin neurulation without a conventional epithelium, medially located neural plate cells adopt strategies typical of epithelia in order to constrict their dorsal surface membrane during cell internalisation. Furthermore, we show that Myosin-II activity is a significant driver of this transient cell remodeling which also depends on Cdh2 (N-cadherin). Abrogation of Cdh2 results in defective Myosin-II distribution, mislocalised internalisation events and defective neural plate morphogenesis. Our work suggests Cdh2 coordinates Myosin-II dependent internalisation of the zebrafish neural plate.
Identifiants
pubmed: 30755665
doi: 10.1038/s41598-018-38455-w
pii: 10.1038/s41598-018-38455-w
pmc: PMC6372647
doi:
Substances chimiques
Actins
0
Cadherins
0
Cdh2 protein, zebrafish
0
Heterocyclic Compounds, 4 or More Rings
0
Oligonucleotides
0
Zebrafish Proteins
0
Green Fluorescent Proteins
147336-22-9
blebbistatin
20WC4J7CQ6
Myosin Type II
EC 3.6.1.-
Myosins
EC 3.6.4.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1835Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 102895/Z/13/Z
Pays : United Kingdom
Commentaires et corrections
Type : ErratumIn
Références
Development. 2002 Jul;129(14):3281-94
pubmed: 12091300
Curr Biol. 2003 Dec 16;13(24):2125-37
pubmed: 14680628
J Muscle Res Cell Motil. 2004;25(4-5):337-41
pubmed: 15548862
Development. 2005 Sep;132(18):4165-78
pubmed: 16123312
J Cell Sci. 2005 Nov 15;118(Pt 22):5191-203
pubmed: 16249236
Nature. 2006 Jan 12;439(7073):220-4
pubmed: 16407953
Development. 2006 Oct;133(19):3895-905
pubmed: 16943271
Nature. 2007 Apr 12;446(7137):797-800
pubmed: 17392791
Nature. 2009 Jan 22;457(7228):495-9
pubmed: 19029882
Dev Biol. 2009 Mar 15;327(2):327-38
pubmed: 19121300
Development. 2009 Apr;136(8):1327-38
pubmed: 19279134
Development. 1991 Jul;112(3):775-89
pubmed: 1935689
Sci Signal. 2009 Apr 14;2(66):ra16
pubmed: 19366994
Curr Opin Neurobiol. 2009 Apr;19(2):134-8
pubmed: 19447605
J Neurosci. 2009 Sep 16;29(37):11426-40
pubmed: 19759292
J Cell Biol. 2010 Mar 8;188(5):735-49
pubmed: 20194639
Development. 2010 Apr;137(8):1315-25
pubmed: 20332149
Nat Cell Biol. 2010 Jun;12(6):533-42
pubmed: 20453849
Curr Biol. 2010 Nov 9;20(21):1966-72
pubmed: 20970340
BMC Biol. 2011 Dec 29;9:90
pubmed: 22206439
Nat Biotechnol. 2012 Jun 24;30(7):708-14
pubmed: 22729030
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12568-73
pubmed: 22802638
Dev Cell. 2012 Sep 11;23(3):533-46
pubmed: 22902739
Science. 2012 Oct 12;338(6104):253-6
pubmed: 22923438
Science. 2012 Oct 12;338(6104):257-60
pubmed: 23066079
Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19298-303
pubmed: 23134725
EMBO J. 2013 Jan 9;32(1):30-44
pubmed: 23202854
Development. 2014 Mar;141(6):1282-91
pubmed: 24595289
Neural Dev. 2014 Apr 23;9:9
pubmed: 24755297
Development. 2014 May;141(10):1987-98
pubmed: 24803648
Nat Commun. 2014 May 13;5:3734
pubmed: 24818582
J Cell Sci. 2015 Jul 15;128(14):2468-81
pubmed: 26040287
Dev Dyn. 2016 Mar;245(3):197-208
pubmed: 26177834
Cell. 2015 Jul 30;162(3):648-61
pubmed: 26232230
Nat Commun. 2016 Feb 25;7:8674
pubmed: 26912388
Dev Dyn. 2016 May;245(5):580-9
pubmed: 26933766
Development. 2017 Feb 15;144(4):552-566
pubmed: 28196803
Bioinformatics. 2017 Aug 1;33(15):2424-2426
pubmed: 28369169
Elife. 2017 May 24;6:
pubmed: 28537220
Development. 2017 Dec 1;144(23):4249-4260
pubmed: 29183938
J Exp Zool. 1985 Aug;235(2):205-15
pubmed: 3903030
Dev Dyn. 1995 Jul;203(3):253-310
pubmed: 8589427
Annu Rev Cell Dev Biol. 1995;11:189-212
pubmed: 8689556
Dev Biol. 1997 Jan 1;181(1):64-78
pubmed: 9015265