Analysis of Epichloë festucae small secreted proteins in the interaction with Lolium perenne.
Agrobacterium tumefaciens
Computational Biology
DNA Transposable Elements
Endophytes
/ genetics
Epichloe
/ genetics
Escherichia coli
Fungal Proteins
/ genetics
Gene Expression Regulation, Fungal
Host Microbial Interactions
/ genetics
Lolium
/ growth & development
Mutation
Saccharomyces cerevisiae
Sequence Analysis
Transcriptome
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
01
12
2018
accepted:
25
01
2019
entrez:
14
2
2019
pubmed:
14
2
2019
medline:
13
11
2019
Statut:
epublish
Résumé
Epichloë festucae is an endophyte of the agriculturally important perennial ryegrass. This species systemically colonises the aerial tissues of this host where its growth is tightly regulated thereby maintaining a mutualistic symbiotic interaction. Recent studies have suggested that small secreted proteins, termed effectors, play a vital role in the suppression of host defence responses. To date only a few effectors with important roles in mutualistic interactions have been described. Here we make use of the fully assembled E. festucae genome and EffectorP to generate a suite of 141 effector candidates. These were analysed with respect to their genome location and expression profiles in planta and in several symbiosis-defective mutants. We found an association between effector candidates and a class of transposable elements known as MITEs, but no correlation with other dynamic features of the E. festucae genome, such as transposable element-rich regions. Three effector candidates and a small GPI-anchored protein were chosen for functional analysis based on their high expression in planta compared to in culture and their differential regulation in symbiosis defective E. festucae mutants. All three candidate effector proteins were shown to possess a functional signal peptide and two could be detected in the extracellular medium by western blotting. Localization of the effector candidates in planta suggests that they are not translocated into the plant cell, but rather, are localized in the apoplastic space or are attached to the cell wall. Deletion and overexpression of the effector candidates, as well as the putative GPI-anchored protein, did not affect the plant growth phenotype or restrict growth of E. festucae mutants in planta. These results indicate that these proteins are either not required for the interaction at the observed life stages or that there is redundancy between effectors expressed by E. festucae.
Identifiants
pubmed: 30759164
doi: 10.1371/journal.pone.0209463
pii: PONE-D-18-34444
pmc: PMC6374014
doi:
Substances chimiques
DNA Transposable Elements
0
Fungal Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0209463Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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