Translocation of Zymomonas mobilis pyruvate decarboxylase to periplasmic compartment for production of acetaldehyde outside the cytosol.
Zymomonas mobilis
acetaldehyde production
periplasm
pyruvate decarboxylase
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
30
11
2018
revised:
11
01
2019
accepted:
12
01
2019
pubmed:
17
2
2019
medline:
17
4
2020
entrez:
17
2
2019
Statut:
ppublish
Résumé
Acetaldehyde, a valuable commodity chemical, is a volatile inhibitory byproduct of aerobic fermentation in Zymomonas mobilis and in several other microorganisms. Attempting to improve acetaldehyde production by minimizing its contact with the cell interior and facilitating its removal from the culture, we engineered a Z. mobilis strain with acetaldehyde synthesis reaction localized in periplasm. For that, the pyruvate decarboxylase (PDC) was transferred from the cell interior to the periplasmic compartment. This was achieved by the construction of a Z. mobilis Zm6 PDC-deficient mutant, fusion of PDC with the periplasmic signal sequence of Z. mobilis gluconolactonase, and the following expression of this fusion protein in the PDC-deficient mutant. The obtained recombinant strain PeriAc, with most of its PDC localized in periplasm, showed a twofold higher acetaldehyde yield, than the parent strain, and will be used for further improvement by directed evolution.
Identifiants
pubmed: 30770675
doi: 10.1002/mbo3.809
pmc: PMC6692523
doi:
Substances chimiques
Recombinant Fusion Proteins
0
Pyruvate Decarboxylase
EC 4.1.1.1
Acetaldehyde
GO1N1ZPR3B
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e00809Informations de copyright
© 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
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