Bacterial Adhesion on Soft Materials: Passive Physicochemical Interactions or Active Bacterial Mechanosensing?


Journal

Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613

Informations de publication

Date de publication:
04 2019
Historique:
received: 18 10 2018
revised: 27 01 2019
pubmed: 19 2 2019
medline: 23 6 2020
entrez: 19 2 2019
Statut: ppublish

Résumé

The influence of mechanical stiffness of biomaterials on bacterial adhesion is only sparsely studied and the mechanism behind this influence remains unclear. Here, bacterial adhesion on polydimethylsiloxane (PDMS) samples, having four different degrees of stiffness with Young's modulus ranging from 0.06 to 4.52 MPa, is investigated. Escherichia coli and Pseudomonas aeruginosa are found to adhere in greater numbers on soft PDMS (7- and 27-fold increase, respectively) than on stiff PDMS, whereas Staphylococcus aureus adheres in similar numbers on the four tested surfaces. To determine whether the observed adhesion behavior is caused by bacteria-specific mechanisms, abiotic polystyrene (PS) beads are employed as bacteria substitutes. Carboxylate-modified PS (PS-COOH) beads exhibit the same adhesion pattern as E. coli and P. aeruginosa with four times more adhered beads on soft PDMS than on stiff PDMS. In contrast, amine-modified PS (PS-NH

Identifiants

pubmed: 30773835
doi: 10.1002/adhm.201801323
doi:

Substances chimiques

Dimethylpolysiloxanes 0
Polystyrenes 0
baysilon 63148-62-9

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1801323

Informations de copyright

© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Auteurs

Hervé Straub (H)

Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science & Technology, Lerchenfeldstrasse 5, 9014, St. Gallen, Switzerland.

Claudio M Bigger (CM)

Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science & Technology, Lerchenfeldstrasse 5, 9014, St. Gallen, Switzerland.

Jules Valentin (J)

Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science & Technology, Lerchenfeldstrasse 5, 9014, St. Gallen, Switzerland.

Dominik Abt (D)

Department of Urology, Cantonal Hospital St. Gallen, Rorschacher Strasse 95, 9007, St. Gallen, Switzerland.

Xiao-Hua Qin (XH)

Institute for Biomechanics, ETH Zürich Leopold-Ruzicka-Weg 4, 8093, Zürich, Switzerland.

Leo Eberl (L)

Department of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, 8008, Zürich, Switzerland.

Katharina Maniura-Weber (K)

Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science & Technology, Lerchenfeldstrasse 5, 9014, St. Gallen, Switzerland.

Qun Ren (Q)

Laboratory for Biointerfaces, Empa, Swiss Federal Laboratories for Materials Science & Technology, Lerchenfeldstrasse 5, 9014, St. Gallen, Switzerland.

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Classifications MeSH