High-efficiency generation of fertile transplastomic Arabidopsis plants.
Arabidopsis
/ cytology
Bacterial Proteins
/ genetics
Biolistics
/ methods
CRISPR-Cas Systems
Cell Culture Techniques
Chloroplasts
/ genetics
Gene Editing
Gene Knockout Techniques
Genetic Vectors
Luminescent Proteins
/ genetics
Plant Roots
/ cytology
Plants, Genetically Modified
Plastids
/ genetics
Transformation, Genetic
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
11
10
2018
accepted:
07
01
2019
pubmed:
20
2
2019
medline:
18
6
2019
entrez:
20
2
2019
Statut:
ppublish
Résumé
The development of technologies for the stable genetic transformation of plastid (chloroplast) genomes has been a boon to both basic and applied research. However, extension of the transplastomic technology to major crops and model plants has proven extremely challenging, and the species range of plastid transformation is still very much limited in that most species currently remain recalcitrant to plastid genome engineering. Here, we report an efficient plastid transformation technology for the model plant Arabidopsis thaliana that relies on root-derived microcalli as a source tissue for biolistic transformation. The method produces fertile transplastomic plants at high frequency when combined with a clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9)-generated knockout allele of a nuclear locus that enhances sensitivity to the selection agent used for isolation of transplastomic events. Our work makes the model organism of plant biology amenable to routine engineering of the plastid genome, facilitates the combination of plastid engineering with the power of Arabidopsis nuclear genetics, and informs the future development of plastid transformation protocols for other recalcitrant species.
Identifiants
pubmed: 30778165
doi: 10.1038/s41477-019-0359-2
pii: 10.1038/s41477-019-0359-2
pmc: PMC6420123
mid: EMS81139
doi:
Substances chimiques
Bacterial Proteins
0
Luminescent Proteins
0
yellow fluorescent protein, Bacteria
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
282-289Commentaires et corrections
Type : CommentIn
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