Development of an interspecies interaction model: An experiment on Clostridium cadaveris and Clostridium sporogenes under anaerobic condition.

Anaerobiosis Carbon Clostridium Real-Time Polymerase Chain Reaction
Biokinetic analysis Clostridium cadaveris Clostridium sporogenes Interspecific interaction model development Primer and probe set Quantitative real-time PCR

Journal

Journal of environmental management
ISSN: 1095-8630
Titre abrégé: J Environ Manage
Pays: England
ID NLM: 0401664

Informations de publication

Date de publication:
01 May 2019
Historique:
received: 01 09 2018
revised: 15 02 2019
accepted: 17 02 2019
pubmed: 25 2 2019
medline: 26 9 2019
entrez: 25 2 2019
Statut: ppublish

Résumé

The specific primer and probe sets for quantifying Clostridium cadaveris and Clostridium sporogenes using a quantitative real-time PCR were designed. Each primer and probe set detected only the target species very specifically. The two species were cultivated in pure and mixed culture in batch mode with glucose as the only carbon source. The designed QPCR sets were used successfully to estimate the biokinetic parameters of each species in pure culture: i.e., maximum specific growth rate μ

Identifiants

DOI: 10.1016/j.jenvman.2019.02.084 PMID: 30798043
pubmed: 30798043
pii: S0301-4797(19)30235-X
doi: 10.1016/j.jenvman.2019.02.084
pii:
doi:

Substances chimiques

Carbon 7440-44-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

247-254

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Taewoan Koo (T)

Division of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea.

Joonyeob Lee (J)

Division of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea.

Seokhwan Hwang (S)

Division of Environmental Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, South Korea. Electronic address: shwang@postech.ac.kr.

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Classifications MeSH

questionsmedicales.fr › Métabolisme › Anaérobiose › Development of an interspecies interaction...
questionsmedicales.fr › Produits chimiques inorganiques › Éléments › Carbone › Development of an interspecies interaction...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bactéries à Gram positif formant des endospores › Clostridium › Development of an interspecies interaction...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Development of an interspecies interaction...