Expression of genes controlling steroid metabolism and action in granulosa-lutein cells of women with polycystic ovaries.
Adult
Androgens
/ pharmacology
Body Mass Index
Cells, Cultured
Dihydrotestosterone
/ pharmacology
Estradiol
/ metabolism
Female
Follicle Stimulating Hormone
/ pharmacology
Gene Expression Regulation
/ drug effects
Gene Regulatory Networks
Granulosa Cells
/ drug effects
Humans
In Vitro Oocyte Maturation Techniques
Luteal Cells
/ drug effects
Models, Biological
Ovulation
/ genetics
Polycystic Ovary Syndrome
/ genetics
Receptors, Estrogen
/ genetics
Reference Standards
Steroids
/ metabolism
Granulosa cells
PCOS
Steroidogenic enzymes
Journal
Molecular and cellular endocrinology
ISSN: 1872-8057
Titre abrégé: Mol Cell Endocrinol
Pays: Ireland
ID NLM: 7500844
Informations de publication
Date de publication:
15 04 2019
15 04 2019
Historique:
received:
19
11
2018
revised:
15
01
2019
accepted:
18
02
2019
pubmed:
26
2
2019
medline:
18
1
2020
entrez:
26
2
2019
Statut:
ppublish
Résumé
Aberrant function of granulosa cells has been implicated in the pathophysiology of PCOS. Granulosa lutein (GL) cells were collected during oocyte retrieval for IVF/ICSI. RT-qPCR was used to compare gene expression between 12 control women, 12 with ovulatory PCO and 12 with anovulatory PCOS. To examine which genes are directly regulated by androgens, GL cells from an additional 12 control women were treated in-vitro with 10 nM dihydrotestosterone (DHT). GL cells from women with PCOS showed reduced expression of CYP11A1 3-fold (p = 0.005), HSD17B1 1.8-fold (p = 0.02) and increased expression of SULT1E1 7-fold (p = 0.0003). Similar results were seen in ovulatory women with PCO. GL cells treated with 10 nM DHT showed a 4-fold (p = 0.03) increase in expression of SULT1E1 and a 5-fold reduction in SRD5A1 (p = 0.03). These findings support the notion that aberrant regulation of steroid metabolism or action play a part in ovarian dysfunction in PCOS.
Identifiants
pubmed: 30802529
pii: S0303-7207(19)30061-9
doi: 10.1016/j.mce.2019.02.015
pii:
doi:
Substances chimiques
Androgens
0
Receptors, Estrogen
0
Steroids
0
Dihydrotestosterone
08J2K08A3Y
Estradiol
4TI98Z838E
Follicle Stimulating Hormone
9002-68-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
47-54Subventions
Organisme : Medical Research Council
ID : G0802782
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M012638/1
Pays : United Kingdom
Informations de copyright
Copyright © 2019. Published by Elsevier B.V.