Species-specific PCR assays for Gambierdiscus excentricus and Gambierdiscus silvae (Gonyaulacales, Dinophyceae).

Caribbean ciguatera ciguatoxin dinoflagellate harmful algae ribosomal species-specific molecular assay

Journal

Journal of phycology
ISSN: 1529-8817
Titre abrégé: J Phycol
Pays: United States
ID NLM: 9882935

Informations de publication

Date de publication:
06 2019
Historique:
received: 18 03 2018
accepted: 28 11 2018
pubmed: 1 3 2019
medline: 23 2 2020
entrez: 1 3 2019
Statut: ppublish

Résumé

The two most toxic Gambierdiscus species identified from the Caribbean are G. excentricus and G. silvae. These species are the primary causes of ciguatera fish poisoning and likely contribute disproportionately to the toxicity of marine food webs. While Gambierdiscus species are difficult to distinguish using light or scanning electron microscopy, reliable species-specific molecular identification methods have been developed and used successfully to identify a number of other Gambierdiscus species. Corresponding species-specific assays are not yet available for G. excentricus and G. silvae, which imposes limitations on species identification and related ecological studies. The following note describes species-specific polymerase chain reaction assays for G. excentricus and G. silvae that can be used for these purposes.

Identifiants

pubmed: 30817008
doi: 10.1111/jpy.12852
doi:

Substances chimiques

Ciguatoxins 11050-21-8

Banques de données

GENBANK
['MG981052', 'MG981061']

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

730-732

Informations de copyright

© 2019 Phycological Society of America.

Auteurs

R Wayne Litaker (RW)

National Oceanic and Atmospheric Administration, National Ocean Service, National Centers for Coastal Ocean Science, Beaufort Laboratory, 101 Pivers Island Rd., Beaufort, North Carolina, 28516, USA.

Patricia A Tester (PA)

Ocean Tester, LLC, 295 Dills Point Road, Beaufort, North Carolina, 28516, USA.

Mark W Vandersea (MW)

National Oceanic and Atmospheric Administration, National Ocean Service, National Centers for Coastal Ocean Science, Beaufort Laboratory, 101 Pivers Island Rd., Beaufort, North Carolina, 28516, USA.

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Classifications MeSH