Genome mining identifies cepacin as a plant-protective metabolite of the biopesticidal bacterium Burkholderia ambifaria.
Animals
Base Sequence
Biological Control Agents
/ metabolism
Burkholderia
/ genetics
Burkholderia cepacia complex
/ genetics
DNA, Bacterial
/ genetics
Disease Models, Animal
Genes, Bacterial
/ genetics
Lactones
/ metabolism
Mice
Multigene Family
Phylogeny
Plant Diseases
/ microbiology
Plasmids
Pythium
/ drug effects
Repressor Proteins
/ classification
Respiratory Tract Infections
/ drug therapy
Soil Microbiology
Trans-Activators
/ classification
Virulence
Journal
Nature microbiology
ISSN: 2058-5276
Titre abrégé: Nat Microbiol
Pays: England
ID NLM: 101674869
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
05
06
2018
accepted:
22
01
2019
pubmed:
6
3
2019
medline:
26
7
2019
entrez:
6
3
2019
Statut:
ppublish
Résumé
Beneficial microorganisms are widely used in agriculture for control of plant pathogens, but a lack of efficacy and safety information has limited the exploitation of multiple promising biopesticides. We applied phylogeny-led genome mining, metabolite analyses and biological control assays to define the efficacy of Burkholderia ambifaria, a naturally beneficial bacterium with proven biocontrol properties but potential pathogenic risk. A panel of 64 B. ambifaria strains demonstrated significant antimicrobial activity against priority plant pathogens. Genome sequencing, specialized metabolite biosynthetic gene cluster mining and metabolite analysis revealed an armoury of known and unknown pathways within B. ambifaria. The biosynthetic gene cluster responsible for the production of the metabolite cepacin was identified and directly shown to mediate protection of germinating crops against Pythium damping-off disease. B. ambifaria maintained biopesticidal protection and overall fitness in the soil after deletion of its third replicon, a non-essential plasmid associated with virulence in Burkholderia cepacia complex bacteria. Removal of the third replicon reduced B. ambifaria persistence in a murine respiratory infection model. Here, we show that by using interdisciplinary phylogenomic, metabolomic and functional approaches, the mode of action of natural biological control agents related to pathogens can be systematically established to facilitate their future exploitation.
Identifiants
pubmed: 30833726
doi: 10.1038/s41564-019-0383-z
pii: 10.1038/s41564-019-0383-z
pmc: PMC6544543
mid: EMS81462
doi:
Substances chimiques
Biological Control Agents
0
DNA, Bacterial
0
Lactones
0
Repressor Proteins
0
Trans-Activators
0
LuxR autoinducer binding proteins
115038-68-1
cepacin A
91682-95-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
996-1005Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 204457
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L021692/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L015080/1
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
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