Synergistic activity of colistin with azidothymidine against colistin-resistant Klebsiella pneumoniae clinical isolates collected from inpatients in Greek hospitals.


Journal

International journal of antimicrobial agents
ISSN: 1872-7913
Titre abrégé: Int J Antimicrob Agents
Pays: Netherlands
ID NLM: 9111860

Informations de publication

Date de publication:
Jun 2019
Historique:
received: 26 10 2018
revised: 08 02 2019
accepted: 26 02 2019
pubmed: 6 3 2019
medline: 3 9 2019
entrez: 6 3 2019
Statut: ppublish

Résumé

New antibiotics are urgently needed to treat multi-drug resistant infections; however, production of novel antibiotics is diminishing. Synergistic combination drug therapy to enhance the activity of available antibiotics may improve management of patients with resistant infections. Colistin-resistant Klebsiella pneumoniae isolates were collected from inpatients in 10 Greek hospitals and used to study combination activity of colistin plus azidothymidine. Combination activity was evaluated with the sum of fractional inhibitory concentrations (ΣFIC) using the mini checkerboard broth microdilution method. A hundred individual strains were tested. Synergistic activity was noted in 79% (79/100) of isolates and additive activity in the remaining 21% (21/100). ΣFIC Colistin with azidothymidine exhibited promising synergistic activity against colistin-resistant Klebsiella pneumoniae isolates warranting further investigation of the combination.

Sections du résumé

BACKGROUND BACKGROUND
New antibiotics are urgently needed to treat multi-drug resistant infections; however, production of novel antibiotics is diminishing. Synergistic combination drug therapy to enhance the activity of available antibiotics may improve management of patients with resistant infections.
METHODS METHODS
Colistin-resistant Klebsiella pneumoniae isolates were collected from inpatients in 10 Greek hospitals and used to study combination activity of colistin plus azidothymidine. Combination activity was evaluated with the sum of fractional inhibitory concentrations (ΣFIC) using the mini checkerboard broth microdilution method.
RESULTS RESULTS
A hundred individual strains were tested. Synergistic activity was noted in 79% (79/100) of isolates and additive activity in the remaining 21% (21/100). ΣFIC
CONCLUSION CONCLUSIONS
Colistin with azidothymidine exhibited promising synergistic activity against colistin-resistant Klebsiella pneumoniae isolates warranting further investigation of the combination.

Identifiants

pubmed: 30836109
pii: S0924-8579(19)30051-2
doi: 10.1016/j.ijantimicag.2019.02.021
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
Zidovudine 4B9XT59T7S
Colistin Z67X93HJG1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

855-858

Informations de copyright

Copyright © 2019 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

Auteurs

Matthew E Falagas (ME)

Alfa Institute of Biomedical Sciences (AIBS), Athens, Greece; Department of Medicine, Henry Dunant Hospital Center, Athens, Greece; Department of Medicine, Tufts University School of Medicine, Boston, MA, USA. Electronic address: m.falagas@aibs.gr.

Georgios L Voulgaris (GL)

Alfa Institute of Biomedical Sciences (AIBS), Athens, Greece; Laboratory of Pharmacokinetics and Toxicology, Department of Pharmacy, 401 General Military Hospital, Athens, Greece.

Kyriaki Tryfinopoulou (K)

Department of Microbiology, National School of Public Health; Central Public Health Laboratory, Hellenic Centre of Disease Control and Prevention, Vari, Greece.

Panagiota Giakkoupi (P)

Department of Microbiology, National School of Public Health; Central Public Health Laboratory, Hellenic Centre of Disease Control and Prevention, Vari, Greece.

Margarita Kyriakidou (M)

Alfa Institute of Biomedical Sciences (AIBS), Athens, Greece.

Alkiviadis Vatopoulos (A)

Department of Microbiology, National School of Public Health; Central Public Health Laboratory, Hellenic Centre of Disease Control and Prevention, Vari, Greece.

Anthony Coates (A)

Institute for Infection and immunity, St. George's, University of London, London, UK.

Yanmin Hu (Y)

Institute for Infection and immunity, St. George's, University of London, London, UK.

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Classifications MeSH